Biotechnologies for Plant Mutation Breeding

This book is open access under a CC BY-NC 2.5 license.

This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.

• TILLING
• Embryonic plant cells
• Mutation detection
• Plant mutagenesis
• Random mutagenesis
• Site-directed mutagenesis
• Mutation induction
• Chimera dissociation
• Phenotypic screening
• Genotypic screening
• genetic engineering

• Plant Breeding and Genetics Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria

  Joanna Jankowicz-Cieslak, Bradley J. Till

• Crops Pathology and Genetics Research Unit, USDA-ARS, Davis, USA

  Thomas H. Tai

• Plant Reproductive Biology, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany

  Jochen Kumlehn

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