Sperm Acrosome Biogenesis and Function During Fertilization

  • Mariano G. Buffone

Part of the Advances in Anatomy, Embryology and Cell Biology book series (ADVSANAT, volume 220)

Table of contents

  1. Front Matter
    Pages i-vii
  2. James A. Foster, George L. Gerton
    Pages 15-33
  3. Carmen Beltrán, Claudia L. Treviño, Esperanza Mata-Martínez, Julio C. Chávez, Claudia Sánchez-Cárdenas, Mark Baker et al.
    Pages 35-69
  4. Silvia A. Belmonte, Luis S. Mayorga, Claudia N. Tomes
    Pages 71-92
  5. Cintia Stival, Lis del C. Puga Molina, Bidur Paudel, Mariano G. Buffone, Pablo E. Visconti, Dario Krapf
    Pages 93-106
  6. Roy Cohen, Chinatsu Mukai, Alexander J. Travis
    Pages 107-127
  7. Ana Romarowski, Guillermina M. Luque, Florenza A. La Spina, Dario Krapf, Mariano G. Buffone
    Pages 129-144
  8. Noritaka Hirohashi
    Pages 145-158
  9. Patricia S. Cuasnicú, Vanina G. Da Ros, Mariana Weigel Muñoz, Débora J. Cohen
    Pages 159-172

About this book

Introduction

Over the last decades, acrosomal exocytosis (also called the “acrosome reaction”) has been recognized as playing an essential role in fertilization. Secretion of this granule is an absolute requirement for physiological fertilization. In recent years, the study of mammalian acrosomal exocytosis has yielded some major advances that challenge the long-held, general paradigms in the field. Principally, the idea that sperm must be acrosome-intact to bind to the zona pellucida of unfertilized eggs, based largely on in vitro fertilization studies of mouse oocytes denuded of the cumulus oophorus, has been overturned by experiments using state-of-the-art imaging of cumulus-intact oocytes and fertilization experiments where eggs were reinseminated by acrosome-reacted sperm recovered from the perivitelline space of zygotes. From a molecular point of view, acrosome exocytosis is a synchronized and tightly regulated process mediated by molecular mechanisms that are homologous to those reported in neuroendocrinal cell secretions. The authors provide a broader perspective, focusing on a limited number of important topics that are essential for understanding the molecular mechanisms governing this step in the fertilization process. They also discuss molecular aspects such as the signaling pathways leading to exocytosis, including the participation of ion channels, lipids, the fusion machinery proteins and the actin cytoskeleton as well as cellular aspects such as the site of acrosomal exocytosis and the use of gene-manipulated animals to study this process.  

Keywords

acrosomal exocytosis fertilization process spermatogenesis acrosome reaction acrosomal matrix sperm capacitation

Editors and affiliations

  • Mariano G. Buffone
    • 1
  1. 1.Instituto de Biologia y Medicina ExperimBuenos AiresArgentina

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-319-30567-7
  • Copyright Information Springer International Publishing Switzerland 2016
  • Publisher Name Springer, Cham
  • eBook Packages Medicine
  • Print ISBN 978-3-319-30565-3
  • Online ISBN 978-3-319-30567-7
  • Series Print ISSN 0301-5556
  • Series Online ISSN 2192-7065
  • About this book