Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis
Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes
Broadens readers’ understanding of microfluidics convection-based PCR technology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics
Includes supplementary material: sn.pub/extras
Table of contents (16 chapters)
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- Daniel A. Porto, Tel M. Rouse, Adriana San-Miguel, Hang Lu
Pages 1-18
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- Arnab Mukherjee, Charles M. Schroeder
Pages 19-54
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- Aashish Priye, Victor M. Ugaz
Pages 55-69
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- Tao Geng, Richard A. Mathies
Pages 71-94
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- Robert J. Meagher, Meiye Wu
Pages 95-112
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- Mei He, Andrew Godwin, Yong Zeng
Pages 113-139
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- Shay Mailloux, Lisa Ramirez, Jun Wang
Pages 141-159
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- A. E. Vasdekis, G. Stephanopoulos
Pages 189-204
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- Chen Zhao, Martin M. Thuo, Xinyu Liu
Pages 205-218
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- Pooja Sabhachandani, Saheli Sarkar, Tania Konry
Pages 219-233
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- Dustin K. Harshman, Jeong-Yeol Yoon
Pages 235-252
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- Elisa M. Wasson, Temple A. Douglas, Rafael V. Davalos
Pages 253-294
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- Caroline N. Jones, Joseph M. Martel-Foley
Pages 295-322
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- Deepika Devadas, Edmond W. K. Young
Pages 323-347
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Back Matter
Pages 365-376
About this book
This book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis. Key molecular biology studies using model organisms are reviewed in detail. This is an ideal book for students and researchers in the microfluidics and molecular biology fields as well as engineers working in the biotechnology industry.
This book also:
- Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis
- Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes
- Broadens readers’ understanding of microfluidics convection-based PCR technology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics
Keywords
- cellular heterogeneity in tissues
- genetic analysis of single cells
- microfluidic PCR
- microfluidic methods for molecular biology
- microfluidic stretching of chromatins
- molecular separations using micro and nanofluidics
- biochemical engineering
Editors and Affiliations
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Virginia Tech, Blacksburg, USA
Chang Lu
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Dpt of Biomedical Engg. & Mechanics, Virginia Tech, Blacksburg, USA
Scott S. Verbridge
About the editors
Chang Lu is a Professor of Chemical Engineering at Virginia Tech. His research focuses on developing microfluidic biotechnologies for cellular and molecular analysis with recent emphasis on low-input epigenomic studies. Scott Verbridge is an Assistant Professor in the Virginia Tech Department of Biomedical Engineering and Mechanics, and the Virginia Tech - Wake Forest School of Biomedical Engineering and Sciences. His research is focused on developing engineered tumor models to better understand cell-microenvironment interactions, and their impact on tumor progression and therapy response.