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  • © 2015

Plant Microtechniques and Protocols

  • The first comprehensive work of microtechniques of botanical specimens

  • Detailed analysis of various microtechnique protocols of plant specimen studies

  • Clear and easy to understand procedures for various specimen analyses Written by leading scholars from around the world

  • Includes supplementary material: sn.pub/extras

Buying options

eBook USD 299.00
Price excludes VAT (USA)
  • ISBN: 978-3-319-19944-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 379.99
Price excludes VAT (USA)
Hardcover Book USD 379.99
Price excludes VAT (USA)

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Table of contents (30 chapters)

  1. Microtechniques Related to Cell and Molecular Biology Studies

    1. Staining Methods for Programmed Cell Death

      • Owen S. D. Wally, Claudio Stasolla
      Pages 323-336
    2. Laser Microdissection of Plant Tissues

      • Jenna L. Millar, Michael G. Becker, Mark F. Belmonte
      Pages 337-350
    3. RNA In situ Hybridization

      • Shalini Mukherjee, Claudio Stasolla, Belay T. Ayele
      Pages 351-370
  2. Preparative Methods for the Study of Wood Anatomy

    1. Front Matter

      Pages 371-371
    2. Collecting and Processing Wood Microcores for Monitoring Xylogenesis

      • Annie Deslauriers, Sergio Rossi, Eryuan Liang
      Pages 417-429
    3. Three-Dimensional Imaging of Cambium and Secondary Xylem Cells by Confocal Laser Scanning Microscopy

      • Satoshi Nakaba, Peter Kitin, Yusuke Yamagishi, Shahanara Begum, Kayo Kudo, Widyanto Dwi Nugroho et al.
      Pages 431-465
  3. Botanical Techniques and Protocols for Archeology and Herbarium Collection

    1. Front Matter

      Pages 467-467
    2. Collection of Plant Remains from Archaeological Contexts

      • Alessandra Celant, Donatella Magri, Francesca Romana Stasolla
      Pages 469-485
    3. Archaeological Wood Preparation

      • Alessandra Celant, Gemma Coccolini
      Pages 487-493
    4. Archaeopalynological Preparation Techniques

      • Donatella Magri, Federico Di Rita
      Pages 495-506
    5. Plant Collection, Identification, and Herbarium Procedures

      • Bonnie Smith, C.C. Chinnappa
      Pages 541-572
  4. Back Matter

    Pages 573-576

About this book

This work gathers common plant microtechniques and updates their procedures using a simple and fully understandable approach.  The text serves as a handy resource for scientists familiar with the protocols and as a guide for the novice, especially students just beginning to learn about various structural methods for the first time.  This book analyzes a range of topics in order to generate cross-talks among scientists in different research disciplines.

The first section of this volume covers the more commonly used embedding methods, with emphasis on the preparative methods for light and electron microscopy. A number of cell biology related protocols are compiled in the second section to showcase the usefulness of various techniques based on different processing and staining methods. The third section highlights some common and recent procedures in wood preparation. The last section includes botanical methods related to archaeological uses of plant materials. A special chapter on field and herbarium procedures is also included to serve as a guide to students interested in plant collection and taxonomic studies.

Keywords

  • archeology
  • cellular and molecular biology studies
  • plant microtechniques
  • staining procedures
  • wood preparative methods
  • Systematic Botany

Editors and Affiliations

  • Department of Biological Sciences, University of Calgary, CALGARY, Canada

    Edward Chee Tak Yeung

  • Department of Plant Sciences, University of Manitoba, Winnipeg, Canada

    Claudio Stasolla

  • Department of Biological Sciences, University of Manitoba, Winnipeg, Canada

    Michael John Sumner

  • Center for Basic Research in Digestive Disease, Mayo Clinic , Rochester, USA

    Bing Quan Huang

About the editors

Edward C. Yeung
Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada

Claudio Stasolla
Department of Plant Science, University of Manitoba, Winnipeg, Manitoba, Canada

Michael J. Sumner
Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

Bing Q. Huang
Mayo Clinic, Rochester, Minnesota, USA

Bibliographic Information

  • Book Title: Plant Microtechniques and Protocols

  • Editors: Edward Chee Tak Yeung, Claudio Stasolla, Michael John Sumner, Bing Quan Huang

  • DOI: https://doi.org/10.1007/978-3-319-19944-3

  • Publisher: Springer Cham

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Springer International Publishing Switzerland 2015

  • Hardcover ISBN: 978-3-319-19943-6

  • Softcover ISBN: 978-3-319-35935-9

  • eBook ISBN: 978-3-319-19944-3

  • Edition Number: 1

  • Number of Pages: XIV, 576

  • Number of Illustrations: 20 b/w illustrations, 68 illustrations in colour

  • Topics: Plant Biotechnology, Plant Development, Plant Evolution

Buying options

eBook USD 299.00
Price excludes VAT (USA)
  • ISBN: 978-3-319-19944-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 379.99
Price excludes VAT (USA)
Hardcover Book USD 379.99
Price excludes VAT (USA)