Skip to main content
  • Book
  • © 2015

Plant Microtechniques and Protocols

  • The first comprehensive work of microtechniques of botanical specimens

  • Detailed analysis of various microtechnique protocols of plant specimen studies

  • Clear and easy to understand procedures for various specimen analyses Written by leading scholars from around the world

  • Includes supplementary material: sn.pub/extras

Buying options

eBook USD 299.00
Price excludes VAT (USA)
  • ISBN: 978-3-319-19944-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 379.99
Price excludes VAT (USA)
Hardcover Book USD 379.99
Price excludes VAT (USA)

This is a preview of subscription content, access via your institution.

Table of contents (30 chapters)

  1. Front Matter

    Pages i-xiv
  2. Fixation, Processing, Embedding and Staining of Botanical Specimens

    1. Front Matter

      Pages 1-1
    2. Chemical and Physical Fixation of Cells and Tissues: An Overview

      • Bing Quan Huang, Edward C. Yeung
      Pages 23-43
    3. Paraffin and Polyester Waxes

      • Claudio Stasolla, Edward C. Yeung
      Pages 45-66
    4. The Glycol Methacrylate Embedding Resins—Technovit 7100 and 8100

      • Edward C. Yeung, Colin K. W. Chan
      Pages 67-82
    5. LR White Acrylic Resin

      • Edward C. Yeung, Bing Quan Huang
      Pages 103-116
    6. Fluorescent Staining of Living Plant Cells

      • Sarah Schoor, Shiu-Cheung Lung, Dustin Sigurdson, Simon D. X. Chuong
      Pages 153-165
    7. Improved Methods for Clearing and Staining of Plant Samples

      • Alexander Lux, Marek Vaculík, Ján Kováč
      Pages 167-178
  3. Microtechniques Related to Cell and Molecular Biology Studies

    1. Front Matter

      Pages 179-179
    2. Protoplast Isolation and Staining

      • Shiu-Cheung Lung, Sarah Schoor, Dustin Sigurdson, Makoto Yanagisawa, Kelly Yeung, Mi Qi Liu et al.
      Pages 197-211
    3. Guiding Principles for Live Cell Imaging of Plants Using Confocal Microscopy

      • Subramanian Sankaranarayanan, Marcus A. Samuel
      Pages 213-224
    4. Abscisic Acid Immunostaining

      • Yung-I Lee, Mei-Chu Chung
      Pages 251-261
    5. Plant Chromosome Preparations and Staining for Light Microscopic Studies

      • Subhash C. Hiremath, C. C. Chinnappa
      Pages 263-286
    6. Chromosome Techniques and FISH

      • Mei-Chu Chung
      Pages 287-309

About this book

This work gathers common plant microtechniques and updates their procedures using a simple and fully understandable approach.  The text serves as a handy resource for scientists familiar with the protocols and as a guide for the novice, especially students just beginning to learn about various structural methods for the first time.  This book analyzes a range of topics in order to generate cross-talks among scientists in different research disciplines.

The first section of this volume covers the more commonly used embedding methods, with emphasis on the preparative methods for light and electron microscopy. A number of cell biology related protocols are compiled in the second section to showcase the usefulness of various techniques based on different processing and staining methods. The third section highlights some common and recent procedures in wood preparation. The last section includes botanical methods related to archaeological uses of plant materials. A special chapter on field and herbarium procedures is also included to serve as a guide to students interested in plant collection and taxonomic studies.

Keywords

  • archeology
  • cellular and molecular biology studies
  • plant microtechniques
  • staining procedures
  • wood preparative methods
  • Systematic Botany

Editors and Affiliations

  • Department of Biological Sciences, University of Calgary, CALGARY, Canada

    Edward Chee Tak Yeung

  • Department of Plant Sciences, University of Manitoba, Winnipeg, Canada

    Claudio Stasolla

  • Department of Biological Sciences, University of Manitoba, Winnipeg, Canada

    Michael John Sumner

  • Center for Basic Research in Digestive Disease, Mayo Clinic , Rochester, USA

    Bing Quan Huang

About the editors

Edward C. Yeung
Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada

Claudio Stasolla
Department of Plant Science, University of Manitoba, Winnipeg, Manitoba, Canada

Michael J. Sumner
Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

Bing Q. Huang
Mayo Clinic, Rochester, Minnesota, USA

Bibliographic Information

  • Book Title: Plant Microtechniques and Protocols

  • Editors: Edward Chee Tak Yeung, Claudio Stasolla, Michael John Sumner, Bing Quan Huang

  • DOI: https://doi.org/10.1007/978-3-319-19944-3

  • Publisher: Springer Cham

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Springer International Publishing Switzerland 2015

  • Hardcover ISBN: 978-3-319-19943-6

  • Softcover ISBN: 978-3-319-35935-9

  • eBook ISBN: 978-3-319-19944-3

  • Edition Number: 1

  • Number of Pages: XIV, 576

  • Number of Illustrations: 20 b/w illustrations, 68 illustrations in colour

  • Topics: Plant Biotechnology, Plant Development, Plant Evolution

Buying options

eBook USD 299.00
Price excludes VAT (USA)
  • ISBN: 978-3-319-19944-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 379.99
Price excludes VAT (USA)
Hardcover Book USD 379.99
Price excludes VAT (USA)