Table of contents (16 chapters)
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- Gerald S. Buller, Robert J. Collins
Pages 43-69
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- Thomas Schönau, Sina Riecke, Andreas Bülter, Kristian Lauritsen
Pages 71-87
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- Thomas Dertinger, Steffen Rüttinger
Pages 89-109
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- Kunihiko Ishii, Takuhiro Otosu, Tahei Tahara
Pages 111-128
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- Anders Barth, Lena Voith von Voithenberg, Don C. Lamb
Pages 129-157
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- Kristin S. Grußmayer, Dirk-Peter Herten
Pages 159-190
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- Maria J. Ruedas-Rama, Jose M. Alvarez-Pez, Luis Crovetto, Jose M. Paredes, Angel Orte
Pages 191-223
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- Rafal Fudala, Ryan M. Rich, Joe Kimball, Ignacy Gryczynski, Sangram Raut, Julian Borejdo et al.
Pages 225-239
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- Ingo Gregor, Matthias Patting
Pages 241-263
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- Narain Karedla, Daja Ruhlandt, Anna M. Chizhik, Jörg Enderlein, Alexey I. Chizhik
Pages 265-281
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- Giuseppe Vicidomini, Ivàn Coto Hernàndez, Alberto Diaspro, Silvia Galiani, Christian Eggeling
Pages 283-301
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- Boris Naydenov, Fedor Jelezko
Pages 303-318
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- Andreas Ahlrichs, Benjamin Sprenger, Oliver Benson
Pages 319-341
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Back Matter
Pages 367-370
About this book
This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Keywords
- Antibunchng
- Coincidence correlation
- Diamond defect centers
- FCS
- FLIM
- FRET
- Fluorescence correlation spectroscopy
- Fluorescence lifetime
- Optical tomography
- Single molecule spectroscopy
- Single photon detectors
- TCSPC
- Time interval analysis
- Time-correlated single photon counting
Reviews
“The book is quite specialised and will likely be mainly of interest to researchers developing new photon counting techniques, fluorescence-based or not. … In addition to the constructors of photon counting systems, it could also be useful to some as a reference for comparing results, which could include those working in the life sciences as well as physicists and chemists.” (David Birch, Analytical and Bioanalytical Chemistry, Vol. 408, 2016)