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  • © 2015

Advanced Photon Counting

Applications, Methods, Instrumentation

Editors:

(view affiliations)
  • A comprehensive review

  • Written by experts

  • Richly illustrated

  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Series on Fluorescence (SS FLUOR, volume 15)

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  • ISBN: 978-3-319-15636-1
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Table of contents (16 chapters)

  1. Front Matter

    Pages i-xii
  2. Single-Photon Detectors for Infrared Wavelengths in the Range 1–1.7 μm

    • Gerald S. Buller, Robert J. Collins
    Pages 43-69
  3. Modern Pulsed Diode Laser Sources for Time-Correlated Photon Counting

    • Thomas Schönau, Sina Riecke, Andreas Bülter, Kristian Lauritsen
    Pages 71-87
  4. Lifetime-Weighted FCS and 2D FLCS: Advanced Application of Time-Tagged TCSPC

    • Kunihiko Ishii, Takuhiro Otosu, Tahei Tahara
    Pages 111-128
  5. MFD-PIE and PIE-FI: Ways to Extract More Information with TCSPC

    • Anders Barth, Lena Voith von Voithenberg, Don C. Lamb
    Pages 129-157
  6. Photon Antibunching in Single Molecule Fluorescence Spectroscopy

    • Kristin S. Grußmayer, Dirk-Peter Herten
    Pages 159-190
  7. FLIM Strategies for Intracellular Sensing

    • Maria J. Ruedas-Rama, Jose M. Alvarez-Pez, Luis Crovetto, Jose M. Paredes, Angel Orte
    Pages 191-223
  8. Multiple-Pulse Pumping with Time-Gated Detection for Enhanced Fluorescence Imaging in Cells and Tissue

    • Rafal Fudala, Ryan M. Rich, Joe Kimball, Ignacy Gryczynski, Sangram Raut, Julian Borejdo et al.
    Pages 225-239
  9. Metal-Induced Energy Transfer

    • Narain Karedla, Daja Ruhlandt, Anna M. Chizhik, Jörg Enderlein, Alexey I. Chizhik
    Pages 265-281
  10. The Importance of Photon Arrival Times in STED Microscopy

    • Giuseppe Vicidomini, Ivàn Coto Hernàndez, Alberto Diaspro, Silvia Galiani, Christian Eggeling
    Pages 283-301
  11. Photon Counting and Timing in Quantum Optics Experiments

    • Andreas Ahlrichs, Benjamin Sprenger, Oliver Benson
    Pages 319-341
  12. Photon Counting in Diffuse Optical Imaging

    • Dirk Grosenick
    Pages 343-365
  13. Back Matter

    Pages 367-370

About this book

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.



Keywords

  • Antibunchng
  • Coincidence correlation
  • Diamond defect centers
  • FCS
  • FLIM
  • FRET
  • Fluorescence correlation spectroscopy
  • Fluorescence lifetime
  • Optical tomography
  • Single molecule spectroscopy
  • Single photon detectors
  • TCSPC
  • Time interval analysis
  • Time-correlated single photon counting

Reviews

“The book is quite specialised and will likely be mainly of interest to researchers developing new photon counting techniques, fluorescence-based or not. … In addition to the constructors of photon counting systems, it could also be useful to some as a reference for comparing results, which could include those working in the life sciences as well as physicists and chemists.” (David Birch, Analytical and Bioanalytical Chemistry, Vol. 408, 2016)

Editors and Affiliations

  • J. Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague 8, Czech Republic

    Peter Kapusta

  • PicoQuant GmbH, Berlin, Germany

    Michael Wahl, Rainer Erdmann

About the editors







Bibliographic Information

  • Book Title: Advanced Photon Counting

  • Book Subtitle: Applications, Methods, Instrumentation

  • Editors: Peter Kapusta, Michael Wahl, Rainer Erdmann

  • Series Title: Springer Series on Fluorescence

  • DOI: https://doi.org/10.1007/978-3-319-15636-1

  • Publisher: Springer Cham

  • eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)

  • Copyright Information: Springer International Publishing Switzerland 2015

  • Hardcover ISBN: 978-3-319-15635-4

  • Softcover ISBN: 978-3-319-35429-3

  • eBook ISBN: 978-3-319-15636-1

  • Series ISSN: 1617-1306

  • Series E-ISSN: 1865-1313

  • Edition Number: 1

  • Number of Pages: XII, 370

  • Number of Illustrations: 56 b/w illustrations, 103 illustrations in colour

  • Topics: Spectroscopy, Laser, Biophysics

Buying options

eBook
USD 229.00
Price excludes VAT (USA)
  • ISBN: 978-3-319-15636-1
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 299.99
Price excludes VAT (USA)
Hardcover Book
USD 399.99
Price excludes VAT (USA)