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  • Book
  • © 2014

Fluorescent Methods for Molecular Motors

  • International experts giving a detailed account of frequently used techniques and applications

  • Interdisciplinary approach covering theory, equipment required for measurements, measurement performance and interpretation of data obtained

  • Helpful resource for researchers and graduate students

  • Includes supplementary material: sn.pub/extras

Part of the book series: Experientia Supplementum (EXS, volume 105)

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eBook USD 129.00
Price excludes VAT (USA)
  • ISBN: 978-3-0348-0856-9
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Hardcover Book USD 169.99
Price excludes VAT (USA)

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Table of contents (13 chapters)

  1. Front Matter

    Pages i-x
  2. Fluorescence and Labelling: How to Choose and What to Do

    • Natalia Fili, Christopher P. Toseland
    Pages 1-24
  3. Fluorescent Biosensors: Design and Application to Motor Proteins

    • Simone Kunzelmann, Claudia Solscheid, Martin R. Webb
    Pages 25-47
  4. Rapid Reaction Kinetic Techniques

    • Christopher P. Toseland, Michael A. Geeves
    Pages 49-65
  5. Fluorescent Methods to Study Transcription Initiation and Transition into Elongation

    • Aishwarya P. Deshpande, Shemaila Sultana, Smita S. Patel
    Pages 105-130
  6. Fluorescence Methods in the Investigation of the DEAD-Box Helicase Mechanism

    • Alexandra Z. Andreou, Dagmar Klostermeier
    Pages 161-192
  7. Use of Fluorescent Techniques to Study the In Vitro Movement of Myosins

    • Christopher Toepfer, James R. Sellers
    Pages 193-210
  8. Fluorescence Tracking of Motor Proteins In Vitro

    • Mark DeWitt, Thomas Schenkel, Ahmet Yildiz
    Pages 211-234
  9. Measuring Transport of Motor Cargos

    • Leslie Conway, Jennifer L. Ross
    Pages 235-252
  10. Measuring Two at the Same Time: Combining Magnetic Tweezers with Single-Molecule FRET

    • Marko Swoboda, Maj Svea Grieb, Steffen Hahn, Michael Schlierf
    Pages 253-276
  11. Using Fluorescence to Study Actomyosin in Yeasts

    • Daniel P. Mulvihill
    Pages 277-298

About this book

This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments.

Keywords

  • fluorescence
  • kinetics
  • molecule motors
  • single molecule

Editors and Affiliations

  • Chromosome Organisation and Dynamics, Max-Planck Institute of Biochemistry, Martinsried, Germany

    Christopher P. Toseland

  • Department of Cellular Physiology, Ludwig-Maximilians-Universität München, Munich, Germany

    Natalia Fili

Bibliographic Information

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • ISBN: 978-3-0348-0856-9
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Hardcover Book USD 169.99
Price excludes VAT (USA)