Skip to main content

Advanced Technologies for Protein Complex Production and Characterization

Volume II

  • Book
  • © 2024


  • Useful companion to the first volume on Protein Complex Production
  • Focuses on the characterization of protein complexes
  • Written by renowned active scientists in the field

Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 1453)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 189.00
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

About this book

This edited volume discusses the identification, discovery, characterization, structure determination and modeling of multicomponent macromolecular complexes, and as such, it fully complements the first volume (ISBN 978-3-319-27214-6), which targeted methods of recombinant production of protein complexes. This book is divided in 8 sections offering a selection of technologies widely used in the characterization of protein and protein-nucleic acid complexes for different purposes and at different scales. From native electrophoresis methods, that are accessible to any reasonably well-equipped laboratory, to the sophisticated setup required for structure determination by cryo-electron microscopy or X-ray crystallography, this book contains a wide variety of clearly explained analytic and preparative approaches, connected with the production techniques developed on the previous volume.


The readers will find an integral connection between this book and the first volume, which ensures a comprehensive and updated discussion of the main topics of the discipline. Taken together, these volumes constitute a cohesive and authoritative source of the research on multicomponent macromolecular complexes. In here, we focus on characterization of protein complexes in the broadest sense, which is not typically covered in other sources. Moreover, all chapters are carefully written by world renowned scientists and active researchers, making this volume ideal, not only as a reference source, but also as a companion book for the daily laboratory work.


This book is aimed for a wide range of scientists, from science students to experienced researchers, working on protein and protein-nucleic acid complexes, who need a thorough understanding of protein production and complex characterization.



Table of contents (14 chapters)

Editors and Affiliations

  • Centro de Investigaciones Biologicas Margarita Salas (CIB-CSIC), Madrid, Spain

    M. Cristina Vega

  • Abvance Biotech SL, Madrid, Spain

    Francisco J. Fernández

About the editors

Dr. M. Cristina Vega leads a research group at the Chemical and Structural Biology Department, part of the Center for Biological Research Margarita Salas (CIB-CSIC) in Madrid, Spain. Her research blends biochemical, biophysical, and structural biology techniques to explore the mechanisms by which pathogens circumvent the human complement system and other immune defenses and to develop innovative diagnostic and therapeutic approaches.


Dr. Francisco J. Fernández, as the CEO of Abvance Biotech SL, a cutting-edge biopharmaceutical company in Madrid, Spain, brings his extensive expertise in structural biology and recombinant protein production and characterization to the forefront. His work is distinguished by a profound interest in applying synthetic and computational biology tools and techniques for therapeutic antibody development. Dr. Fernández’s innovative approach in these fields underpins his leadership in advancing biotechnological applications in medicine.

Bibliographic Information

  • Book Title: Advanced Technologies for Protein Complex Production and Characterization

  • Book Subtitle: Volume II

  • Editors: M. Cristina Vega, Francisco J. Fernández

  • Series Title: Advances in Experimental Medicine and Biology

  • DOI:

  • Publisher: Springer Cham

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2024

  • Hardcover ISBN: 978-3-031-52192-8Published: 21 March 2024

  • Softcover ISBN: 978-3-031-52195-9Due: 04 April 2025

  • eBook ISBN: 978-3-031-52193-5Published: 20 March 2024

  • Series ISSN: 0065-2598

  • Series E-ISSN: 2214-8019

  • Edition Number: 1

  • Number of Pages: X, 213

  • Number of Illustrations: 5 b/w illustrations, 45 illustrations in colour

  • Topics: Life Sciences, general

Publish with us