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Table of contents (4 chapters)
About this book
Dielectrophoresis microfluidic chips have been widely used in various biological applications due to their advantages of convenient operation, high throughput, and low cost.
However, most of the DEP microfluidic chips are based on 2D planar electrodes which have some limitations, such as electric field attenuation, small effective working regions, and weak DEP forces. In order to overcome the limitations of 2D planar electrodes, two kinds of thick-electrode DEP chips were designed to realize manipulation and multi-parameter measurement of single cells.
Based on the multi-electrode structure of thick-electrode DEP, a single-cell 3D electro-rotation chip of "Armillary Sphere" was designed. The chip uses four thick electrodes and a bottom planar electrode to form an electric field chamber, which can control 3D rotation of single cells under different electric signal configurations. Electrical property measurement and 3D image reconstruction of single cells are achieved based on single-cell 3D rotation. This work overcomes the limitations of 2D planar electrodes and effectively solves the problem of unstable spatial position of single-cell samples, and provides a new platform for single-cell analysis.
Based on multi-electrode structure of thick-electrode DEP, a microfluidic chip with optoelectronic integration was presented. A dual-fiber optical stretcher embedded in thick electrodes can trap and stretch a single cell while the thick electrodes are used for single-cell rotation. Stretching and rotation manipulation gives the chip the ability to simultaneously measure mechanical and electrical properties of single cells, providing a versatile platform for single-cell analysis, further extending the application of thick-electrode DEP in biological manipulation and analysis.
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Bibliographic Information
Book Title: 3D Electro-Rotation of Single Cells
Authors: Liang Huang, Wenhui Wang
Series Title: Synthesis Lectures on Biomedical Engineering
DOI: https://doi.org/10.1007/978-3-031-01666-0
Publisher: Springer Cham
eBook Packages: Synthesis Collection of Technology (R0), eBColl Synthesis Collection 9
Copyright Information: Springer Nature Switzerland AG 2020
Softcover ISBN: 978-3-031-00538-1Published: 26 December 2019
eBook ISBN: 978-3-031-01666-0Published: 31 May 2022
Series ISSN: 1930-0328
Series E-ISSN: 1930-0336
Edition Number: 1
Number of Pages: XVII, 101
Topics: Engineering, general, Biological and Medical Physics, Biophysics, Biomedical Engineering and Bioengineering