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Laboratory Protocols in Fungal Biology

Current Methods in Fungal Biology

  • Features the work of the foremost authorities on fungal biology

  • Offers information for a diverse audience including both early stage and experienced researchers

  • Discusses protocols derived from current peer-reviewed literature

Part of the book series: Fungal Biology (FUNGBIO)

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eBook USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-3-030-83749-5
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  • Tax calculation will be finalised during checkout
Hardcover Book USD 179.99
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Table of contents (12 chapters)

  1. Front Matter

    Pages i-vi
  2. Various Methods of Long-Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM)

    • Svetlana M. Ozerskaya, Nataliya E. Ivanushkina, Galina A. Kochkina, Anastasya A. Danilogorskaya, Irina P. Pinchuk, Alexander N. Vasilenko
    Pages 1-67
  3. Sabouraud Agar and Other Fungal Growth Media

    • Tankeshwar Acharya, Janelle Hare
    Pages 69-86
  4. Fluorescence In Situ Hybridization of Uncultured Zoosporic Fungi

    • Télesphore Sime-Ngando, Marlène Jobard, Serena Rasconi
    Pages 87-94
  5. Assessment of Host Immune Responses to Fungal Pathogens

    • Huilin Su, Chunxiao Li, Jiande Han, Clement K. M. Tsui, Min Zhu
    Pages 103-116
  6. Recent Advances in Applications of Support Vector Machines in Fungal Biology

    • Sonal Modak, Ashwin Lahorkar, Jayaraman Valadi
    Pages 117-136
  7. Real-Time Quantitative PCR Assay for the Assessment of Uncultured Zoosporic Fungi

    • Télesphore Sime-Ngando, Marlène Jobard
    Pages 137-144
  8. Assays for the Quantification of Antioxidant Enzymes in Fungi

    • Konstantinos Grintzalis, Ioannis Papapostolou, Christos D. Georgiou
    Pages 145-157
  9. Cellulomics of Live Yeast by Advanced and Correlative Microscopy

    • Zinnat Shahina, Supriya V. Bhat, Easter Ndlovu, Taranum Sultana, André Körnig, Étienne Dague et al.
    Pages 159-174
  10. Molecular Taxonomy and Multigene Phylogeny of Filamentous Fungi

    • Nikita Mehta, Reshma Jadhav, Abhishek Baghela
    Pages 175-207
  11. Fluorochrome-Based Methods for Fungal Sample Examination

    • Silvino Intra Moreira, Lucas Fidelis Pereira, Elaine Aparecida de Souza, Eduardo Alves
    Pages 209-234
  12. Yeast Isolation Methods from Specialized Habitats

    • Rameshwar Avchar, Snigdha Tiwari, Abhishek Baghela
    Pages 235-254
  13. Back Matter

    Pages 255-261

About this book

Mycology has an integral role to play in the development of the biotechnology and biomedical sectors. It has become a subject of increasing importance as new fungi and their associated biomolecules are identified. As this discipline comes to the forefront of research in these sectors, the requirement for a consolidation of available research approaches is required. The First Edition of this book has a few basic and applied protocols. With the Second Edition, this book provides consolidated information on recent developments and the most widely used mycological methods available in the fields of biochemistry, biotechnology and microbiology. The methods outlined offer clear and concise directions to the reader and covers both standard protocols and more applied mycological methods. This book provides useful information for undergraduates, post-graduates, and specialists and researchers studying fungal biology.


  • Fungal Biology
  • Mycology
  • Plant Sciences
  • Gene and PCR
  • Biotechnology
  • Microscopy
  • Enzymes
  • Diagnostics

Editors and Affiliations

  • Center for Safe and Improved Food & Biorefining and Advanced Materials Research Center, SRUC, Edinburgh, Scotland, UK

    Vijai Kumar Gupta

  • Department of Biochemistry, National University of Ireland Galway, Galway, Ireland

    Maria Tuohy

About the editors

Vijai Kumar Gupta is currently working as Senior Fellow at Center for Safe and Improved Foods & Biorefining and Advanced Materials Research Center, Scotland’s Rural College (SRUC), Edinburgh, UK. Dr Gupta received his doctoral degree in Microbiology in 2009 from Dr RML Avadh University India, and the leading expert in the area of Biomass Valorisation, Microbial Bioprocess Biotechnologies, Bioactive Natural Products, and Environmental Sustainability.

He is the Secretary of the European Mycological Association and also the International Professor at IFST-China, Visiting Professor at SCBG-China; SZIU-Hungary; SHUATS-India. Dr Gupta is the Fellow of few international scientific societies including- Linnaean Society-UK; Mycological Society of India; Indian Mycological Association; Academy of Microbiological Sciences India; National Academy of Agricultural Sciences, India and Member of Royal Society Biology, UK. He has 175 peer-reviewed publications, 33 book chapters and 39 books in his hands with author h-index 50, i10-index 173 with total 11K+ citations to-date. Also, he is the editor of few reputed journals including, Microbial Cell Factories, Scientific Reports, Microbiome, Biotechnology Advances and Critical Reviews in Biotechnology.

Maria Tuohy is the Principal Investigator and Head of the Molecular Glycobiotechnology Group, School of Natural Sciences, NUI, Galway, Ireland. Dr. Tuohy has a strong track record in Glycobiotechnology and Enzyme Biotechnology, with ~28 years’ experience in fungal biology and biotechnology. A special focus of the research is on developing enabling technology for bioconversion of terrestrial plants and algae for biorefinery and bioenergy applications. This research encompasses protein biotechnology, molecular enzymology, proteomics, fungal genetics, biology and genomics, fermentation, analytical methods in glycobiology and protein biochemistry, development of novel energy systems, as well as key analytical methods to measure and characterize novel bioactive compounds from microbial, plant and marine sources. Understanding fungal biodiversity and the relationships between fungi and other organisms (terrestrial plants and marine life) forms a central theme that underpins the research on developing fungi as cell factories for production of enzymes and bioactives of biotechnological relevance. Research to date has involved ongoing collaboration with industry. Dr. Tuohy is one of 5 PIs in the EI-IDA supported, industry-led, national Technology Centre for Biorefining and Bioenergy (TCBB) and more recently, TCBB Resource. Through TCBB is involved in the European Bio- based Industries Consortium (BIC) and was a PI on the Technological Innovation work package of an EU FP7 INTERREG IVB NWE Programme (‘ReNEW’), which was coordinated by The Queens University Belfast and involved a consortium of RTD, Industry and regional development organizations across NW Europe. Dr. Tuohy is also a co-PI on Pillar 5 of the recent national Dairy Processing Technology Centre (DPTC; €25m funding), since February 2015, and a collaborating PI in the recent SFI- Industry supported ‘Sustainable Energy & Fuel Efficiency Spoke’ in MaREI Research Centre. Dr. Tuohy & her group are members of the Energy Research Centre and Environmental Technologies PRA group, Ryan Institute, NUI Galway, Ireland. Dr. Tuohy has successfully mentored 33 PhD students to completion to-date, and was awarded a prestigious President’s Award for Teaching Excellence in 2011-2012 by NUI Galway, Ireland.

Bibliographic Information

  • Book Title: Laboratory Protocols in Fungal Biology

  • Book Subtitle: Current Methods in Fungal Biology

  • Editors: Vijai Kumar Gupta, Maria Tuohy

  • Series Title: Fungal Biology

  • DOI:

  • Publisher: Springer Cham

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022

  • Hardcover ISBN: 978-3-030-83748-8

  • eBook ISBN: 978-3-030-83749-5

  • Series ISSN: 2198-7777

  • Series E-ISSN: 2198-7785

  • Edition Number: 2

  • Number of Pages: VI, 261

  • Number of Illustrations: 9 b/w illustrations, 15 illustrations in colour

  • Topics: Fungi, Microbiology, Plant Biochemistry, Biotechnology, Plant Development

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-3-030-83749-5
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Hardcover Book USD 179.99
Price excludes VAT (USA)