Human Cytomegaloviruses

Methods and Protocols

  • Andrew D. Yurochko
  • William E. Miller

Part of the Methods in Molecular Biology book series (MIMB, volume 1119)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Maciej T. Nogalski, Donna Collins-McMillen, Andrew D. Yurochko
    Pages 15-28
  3. Iryna Dekhtiarenko, Luka Čičin-Šain, Martin Messerle
    Pages 59-79
  4. Gary C. T. Chan, Andrew D. Yurochko
    Pages 113-121
  5. Christine M. O’Connor, William E. Miller
    Pages 133-164
  6. Charles S. Cobbs, Lisa Matlaf, Lualhati E. Harkins
    Pages 165-196
  7. Craig N. Kreklywich, Patricia P. Smith, Carmen Baca Jones, Anda Cornea, Susan L. Orloff, Daniel N. Streblow
    Pages 217-237
  8. Lauren M. Hook, Igor Landais, Meaghan H. Hancock, Jay A. Nelson
    Pages 239-265
  9. Pranay Dogra, Tim E. Sparer
    Pages 267-288
  10. Djurdjica Cekinovic, Vanda Juranic Lisnic, Stipan Jonjic
    Pages 289-310
  11. Back Matter
    Pages 365-368

About this book


With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed.  Human Cytomegaloviruses: Methods and Protocols is designed to be an inclusive document covering all of the techniques and approaches necessary to understand and study the pathobiology of human cytomegalovirus. Topics covered include the history of human cytomegaloviruses, techniques to culture and grow the virus in model cell types, the use of primary cells for the study of human cytomegalovirus pathogenesis, modern molecular techniques for assessing the biological consequences of viral infection, animal models for study of cytomegalovirus replication and numerous other topics of current interest. Written in the successful Methods in Molecular Biology series format, each chapter includes an introduction, list of necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Human Cytomegaloviruses: Methods and Protocols serves as a tool for basic scientists as well as clinical scientists with an interest in the basic fundamental aspects of viral gene expression and specific aspects of viral pathogenesis. 


cytomegalovirus fibroblasts human cytomegaloviruses pathobiology pathogenesis

Editors and affiliations

  • Andrew D. Yurochko
    • 1
  • William E. Miller
    • 2
  1. 1.Department of Microbiology & ImmunologyLouisiana State University Health SciencShreveportUSA
  2. 2.Department of Molecular GeneticsUniversity of Cincinnati College of MediCincinnatiUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-787-7
  • Online ISBN 978-1-62703-788-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site