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Electron Microscopy

Methods and Protocols

  • Book
  • © 2014
  • Latest edition


  • Deals with biological and molecular research techniques based on TEM and SEM
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1117)

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Table of contents (34 protocols)


About this book

This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.


From the book reviews:

“The amount of information in this book is impressive. … The layout of the book is well-organized and the protocols with their accompanying notes are particularly useful and comprehensive. … I would highly recommend this book to any EM laboratory that does not already have the second edition and to larger EM research facilities that are performing the latest correlative microscopy and image analysis techniques.” (Kath White, Microscopy and Microanalysis, Vol. 20 (5), October, 2014)

Editors and Affiliations

  • University of Western Australia, Crawley, Australia

    John Kuo

Bibliographic Information

  • Book Title: Electron Microscopy

  • Book Subtitle: Methods and Protocols

  • Editors: John Kuo

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-62703-775-4Published: 20 December 2013

  • Softcover ISBN: 978-1-4939-6073-6Published: 27 August 2016

  • eBook ISBN: 978-1-62703-776-1Published: 20 December 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XVIII, 799

  • Number of Illustrations: 156 b/w illustrations, 100 illustrations in colour

  • Topics: Imaging / Radiology

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