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DNA Cloning and Assembly Methods

  • Book
  • © 2014


  • Includes methods based on mechanisms
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1116)

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About this book

In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination.

With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide scientist with a valuable and useful resource for wet lab researchers within life sciences.

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Table of contents (18 protocols)

Editors and Affiliations

  • Norwegian University of Science and Technology, Trondheim, Norway

    Svein Valla, Rahmi Lale

Bibliographic Information

  • Book Title: DNA Cloning and Assembly Methods

  • Editors: Svein Valla, Rahmi Lale

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-62703-763-1Published: 07 January 2014

  • Softcover ISBN: 978-1-4939-6057-6Published: 27 August 2016

  • eBook ISBN: 978-1-62703-764-8Published: 06 January 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 303

  • Number of Illustrations: 24 b/w illustrations, 50 illustrations in colour

  • Topics: Human Genetics, Gene Function

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