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DNA Cloning and Assembly Methods

  • Svein Valla
  • Rahmi Lale

Part of the Methods in Molecular Biology book series (MIMB, volume 1116)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Gunvor Røkke, Eirin Korvald, Jarle Pahr, Ove Øyås, Rahmi Lale
    Pages 1-24
  3. Frank Thieme, Sylvestre Marillonnet
    Pages 37-48
  4. Jonathan L. Schmid-Burgk, Zhen Xie, Yaakov Benenson
    Pages 49-58
  5. Bo Salomonsen, Uffe H. Mortensen, Barbara A. Halkier
    Pages 59-72
  6. Ariel Erijman, Julia M. Shifman, Yoav Peleg
    Pages 89-101
  7. Jiayuan Quan, Jingdong Tian
    Pages 103-117
  8. Carola Engler, Sylvestre Marillonnet
    Pages 119-131
  9. Alejandro Sarrion-Perdigones, Jorge Palaci, Antonio Granell, Diego Orzaez
    Pages 133-151
  10. Mikiko Nakamura, Ayako Suzuki, Hisashi Hoshida, Rinji Akada
    Pages 165-181
  11. Vanessa E. Wall, Leslie A. Garvey, Jennifer L. Mehalko, Lauren V. Procter, Dominic Esposito
    Pages 193-208
  12. Louise E. Bird, Heather Rada, John Flanagan, Jonathan M. Diprose, Robert J. C. Gilbert, Raymond J. Owens
    Pages 209-234
  13. Yongwei Zhang, Uwe Werling, Winfried Edelmann
    Pages 235-244
  14. Nathan J. Hillson
    Pages 245-269
  15. Ruslan Kalendar, David Lee, Alan H. Schulman
    Pages 271-302
  16. Back Matter
    Pages 303-303

About this book

Introduction

In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination.

With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide  scientist with a valuable and useful resource for wet lab researchers within life sciences.

Keywords

DNA cloning DNA fragments II restriction enzymes IIS restriction enzymes cloning genes protein expression sequence overlap single stranded annealing

Editors and affiliations

  • Svein Valla
    • 1
  • Rahmi Lale
    • 2
  1. 1.Norwegian University of Science and TechnologyTrondheimNorway
  2. 2.Norwegian University of Science and TechnologyTrondheimNorway

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-764-8
  • Copyright Information Springer Science+Business Media, New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-763-1
  • Online ISBN 978-1-62703-764-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site