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The Complement System

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Mihaela Gadjeva

    1. Division of Infectious Diseases, Brigham and Women’s Hospital, Harvard University Harvard Medical School, Boston, USA

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  • Presents current up-to-date methodologies
  • Features tips on trouble shooting and avoiding known pitfalls
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1100)

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Table of contents (32 protocols)

  1. Anti-Factor H Autoantibodies Assay

    • Marie-Agnès Dragon-Durey, Caroline Blanc, Lubka T. Roumenina, Nelly Poulain, Stéphanie Ngo, Pauline Bordereau et al.
    Pages 249-256

  2. Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage

    • Najmunisa Karbani, Eswari Dodagatta-Marri, Asif S. Qaseem, Priyaa Madhukaran, Patrick Waters, Anthony G. Tsolaki et al.
    Pages 257-272

  3. Purification of Surfactant Protein D (SP-D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage

    • Eswari Dodagatta-Marri, Asif S. Qaseem, Najmunisa Karbani, Anthony G. Tsolaki, Patrick Waters, Taruna Madan et al.
    Pages 273-290

  4. Functional Analysis of C5a Effector Responses In Vitro and In Vivo

    • Christian M. Karsten, Yves Laumonnier, Jörg Köhl
    Pages 291-304

  5. Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells Using Flow Cytometry

    • Luke R. Donius, John H. Weis
    Pages 305-310

  6. Quantification of Complement Receptor 2 Calcium Signaling Enhancement Using Flow Cytometry

    • Luke R. Donius, John H. Weis
    Pages 311-317

  7. Purification of C1q Receptors and Functional Analysis

    • Berhane Ghebrehiwet, Ellinor I. B. Peerschke
    Pages 319-327

  8. Detection of Cell Membrane-Bound CD46 Using Flow Cytometry

    • Martin Kolev, Claudia Kemper
    Pages 329-339

  9. Detection of CD59 Using Flow Cytometry

    • Martin Kolev
    Pages 341-346

  10. Functional Analysis of CD59 Using Complement-Dependent Cytotoxicity Assay

    • Martin Kolev
    Pages 347-353

  11. Detection of Soluble CR3 (CD11b/CD18) by Time-Resolved Immunofluorometry

    • Gitte Krogh Nielsen, Thomas Vorup-Jensen
    Pages 355-364

  12. Analysis of the Complement Activation in Mice

    • Nirmal K. Banda, Kazue Takahashi
    Pages 365-371

  13. Opsonophagocytic Assay

    • Markryan Dwyer, Mihaela Gadjeva
    Pages 373-379

  14. Back Matter

    Pages 381-383
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Keywords

About this book

Complement Systems: Methods and Protocols is composed of 32 individual chapters that describe a variety of protocols  to purify and analyze the activity of the individual complement components or pathways.  It includes assays that describe detection  of complement SNPs,  clinical methods to evaluate complement system activation and data interpretation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Complement Systems: Methods and Protocols provides a collection of well-established “classical” assays and recently developed “new” assays to analyze the complement system activation will be useful to a wide audience of scientists.

Editors and Affiliations

  • Division of Infectious Diseases, Brigham and Women’s Hospital, Harvard University Harvard Medical School, Boston, USA

    Mihaela Gadjeva

Bibliographic Information

  • Book Title: The Complement System

  • Book Subtitle: Methods and Protocols

  • Editors: Mihaela Gadjeva

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-724-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-62703-723-5Published: 12 November 2013

  • Softcover ISBN: 978-1-4939-6306-5Published: 23 August 2016

  • eBook ISBN: 978-1-62703-724-2Published: 11 November 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 383

  • Number of Illustrations: 54 b/w illustrations, 27 illustrations in colour

  • Topics: Immunology, Antibodies

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