Human Retroviruses

Methods and Protocols

  • Elisa Vicenzi
  • Guido Poli

Part of the Methods in Molecular Biology book series (MIMB, volume 1087)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Mapping the HIV Life Cycle

    1. Front Matter
      Pages 1-1
    2. Yang Yang, Jeremy Luban, Felipe Diaz-Griffero
      Pages 29-36
    3. Anna Cereseto, Mauro Giacca
      Pages 47-54
    4. Andrea Cimarelli, Jean-Luc Darlix
      Pages 55-70
    5. Karin Moelling, Felix Broecker, John E. Kerrigan
      Pages 71-84
    6. Laurence Colin, Eric Verdin, Carine Van Lint
      Pages 85-101
    7. Anke Heigele, Daniel Sauter, Jan Münch, Frank Kirchhoff
      Pages 115-123
    8. Richard Y. Zhao, Michael I. Bukrinsky
      Pages 125-134
    9. Amy Andrew, Klaus Strebel
      Pages 135-158
    10. Barbara Müller, Jacomine Krijnse-Locker
      Pages 167-184
  3. Isolation, Co-Receptor Use, and Cell Tropism of HIV-1

    1. Front Matter
      Pages 185-185
    2. Stefania Dispinseri, Elisa Saba, Elisa Vicenzi, Neeltje A. Kootstra, Hanneke Schuitemaker, Gabriella Scarlatti
      Pages 187-196
    3. Mariangela Cavarelli, Gabriella Scarlatti
      Pages 197-206
    4. Najla Nasr, Andrew Harman, Stuart Turville, Anthony L. Cunningham
      Pages 221-232
    5. Anush Arakelyan, Wendy Fitzgerald, Jean-Charles Grivel, Christophe Vanpouille, Leonid Margolis
      Pages 233-248
  4. In Vivo Quantification of HIV-1

    1. Front Matter
      Pages 249-249
    2. Ann Wiegand, Frank Maldarelli
      Pages 251-260
    3. Christine Rouzioux, Adeline Mélard, Véronique Avéttand-Fénoël
      Pages 261-270
  5. Biotechnological Aspects of HIV-1

    1. Front Matter
      Pages 271-271
    2. Ying Poi Liu, Ben Berkhout
      Pages 273-284
  6. HTLVs

  7. Back Matter
    Pages 339-345

About this book


Human Retroviruses: Methods and Protocols collects key experimental protocols that have provided the basis of the major discoveries of the field. Split into five sections, this detailed volume covers mapping of the HIV life cycle, isolation, co-receptor use, and cell tropism of HIV-1, in vivo quantification of HIV-1, biological aspects of HIV-1, as well as HTLVs. Some articles explore “assay and function of accessory genes”, largely involving the interface between retroviral and host factors, the extracellular role of Tat and Tax, resembling the function of cytokines, and the biotechnological exploitation of HIV as lentiviral vector to carry foreign genes with therapeutic value.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and authoritative, Human Retroviruses: Methods and Protocols provides state-of-art methodological protocols from world leaders in human retrovirology, essential for any lab working this vital field.


Accessory genes HIV life cycle mapping HIV/AIDS HTLVs Retroviral and host factors Retrovirology Tat and Tax

Editors and affiliations

  • Elisa Vicenzi
    • 1
  • Guido Poli
    • 2
  1. 1.Div. Immunology, Transplantation, and Infectious DiseasesSan Raffaele Scientific InstituteMilanoItaly
  2. 2.Div. Immunology, Transplantation, and Infectious DiseasesSan Raffaele Scientific InstituteMilanoItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-669-6
  • Online ISBN 978-1-62703-670-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site