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Neuronal Cell Culture

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Shohreh Amini

    1. Department of Biology, Temple University College of Science & Technology, Philadelphia, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Martyn K. White

    1. Department of Neuroscience, Temple University School of Medicine, Philadelphia, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Covers the latest aspects of the culture of neural cells
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1078)

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About this book

In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved.  Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques.  Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. General Overview of Neuronal Cell Culture

    • Jennifer Gordon, Shohreh Amini, Martyn K. White
    Pages 1-8

  3. Considerations for the Use of SH-SY5Y Neuroblastoma Cells in Neurobiology

    • Jane Kovalevich, Dianne Langford
    Pages 9-21

  4. Cultured Cell Line Models of Neuronal Differentiation: NT2, PC12

    • Nune Darbinian
    Pages 23-33

  5. Murine Teratocarcinoma-Derived Neuronal Cultures

    • Prasun K. Datta
    Pages 35-44

  6. Isolation and Propagation of Primary Human and Rodent Embryonic Neural Progenitor Cells and Cortical Neurons

    • Armine Darbinyan, Rafal Kaminski, Martyn K. White, Nune Darbinian, Kamel Khalili
    Pages 45-54

  7. Mouse Enteric Neuronal Cell Culture

    • Yonggang Zhang, Wenhui Hu
    Pages 55-63

  8. Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications

    • Joseph F. Bonner, Christopher J. Haas, Itzhak Fischer
    Pages 65-88

  9. Derivation of Neuronal Cells from Fetal Normal Human Astrocytes (NHA)

    • S. Ausim Azizi, Barbara Krynska
    Pages 89-96

  10. Slice Culture Modeling of Central Nervous System (CNS) Viral Infection

    • Kalen R. Dionne, Kenneth L. Tyler
    Pages 97-117

  11. Neurospheres and Glial Cell Cultures: Immunocytochemistry for Cell Phenotyping

    • Amanda Parker Struckhoff, Luis Del Valle
    Pages 119-132

  12. Transfection of Neuronal Cultures

    • Ilker Kudret Sariyer
    Pages 133-139

  13. Lentiviral Transduction of Neuronal Cells

    • Hassen S. Wollebo, Baheru Woldemichaele, Martyn K. White
    Pages 141-146

  14. Compartmentalized Neuronal Cultures

    • Armine Darbinyan, Paul Pozniak, Nune Darbinian, Martyn K. White, Kamel Khalili
    Pages 147-152

  15. Quantitative Assessment of Neurite Outgrowth Over Growth Promoting or Inhibitory Substrates

    • George M. Smith, Yingpeng Liu, Jee W. Hong
    Pages 153-161

  16. Back Matter

    Pages 163-165
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Editors and Affiliations

  • Department of Biology, Temple University College of Science & Technology, Philadelphia, USA

    Shohreh Amini

  • Department of Neuroscience, Temple University School of Medicine, Philadelphia, USA

    Martyn K. White

Bibliographic Information

  • Book Title: Neuronal Cell Culture

  • Book Subtitle: Methods and Protocols

  • Editors: Shohreh Amini, Martyn K. White

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-640-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-639-9Published: 24 August 2013

  • Softcover ISBN: 978-1-4939-6064-4Published: 23 August 2016

  • eBook ISBN: 978-1-62703-640-5Published: 24 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 165

  • Number of Illustrations: 7 b/w illustrations, 31 illustrations in colour

  • Topics: Neurosciences, Neurobiology, Cell Culture

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