Fluorescent Protein-Based Biosensors

Methods and Protocols

  • Jin Zhang
  • Qiang Ni
  • Robert H Newman

Part of the Methods in Molecular Biology book series (MIMB, volume 1071)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Alessandra Stangherlin, Andreas Koschinski, Anna Terrin, Anna Zoccarato, He Jiang, Laura Ashley Fields et al.
    Pages 59-71
  3. Adam E. Cohen, Daniel R. Hochbaum
    Pages 97-108
  4. Maya T. Kunkel, Alexandra C. Newton
    Pages 129-137
  5. Marina Polito, Pierre Vincent, Elvire Guiot
    Pages 175-194
  6. Katie Herbst Robinson, Jessica R. Yang, Jin Zhang
    Pages 217-225
  7. Eric C. Greenwald, Renata K. Polanowska-Grabowska, Jeffrey J. Saucerman
    Pages 227-248
  8. Back Matter
    Pages 249-251

About this book

Introduction

In Fluorescent Protein-Based Biosensors: Methods and Protocols, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction.  Genetically encodable fluorescent biosensors have allowed researchers to observe biochemical processes within the endogenous cellular environment with unprecedented spatiotemporal resolution. As the number and diversity of available biosensors grows, it is increasingly important to equip researchers with an understanding of the key concepts underlying the design and application of genetically encodable fluorescent biosensors to live cell imaging. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Fluorescent Protein-Based Biosensors: Methods and Protocols promises to be a valuable resource for researchers interested in applying current biosensors to the study of biochemical processes in living cells as well as those interested in developing novel biosensors to visualize other cellular phenomena.

Keywords

FRET indicators biosensors fluorescent proteins kinase activity live cell imaging

Editors and affiliations

  • Jin Zhang
    • 1
  • Qiang Ni
    • 2
  • Robert H Newman
    • 3
  1. 1.Dept. of Pharmacology & Molecular SciencJohns Hopkins UniversityBaltimoreUSA
  2. 2.Dept. of Pharmacology & Molecular SciencJohns Hopkins UniversityBaltimoreUSA
  3. 3.Department of BiologyNorth Caroline A&T State UnivGreensboroUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-622-1
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-621-4
  • Online ISBN 978-1-62703-622-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book