Methods and Protocols

  • Kelly M. Fulton
  • Susan M. Twine

Part of the Methods in Molecular Biology book series (MIMB, volume 1061)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Scott McComb, Aude Thiriot, Lakshmi Krishnan, Felicity Stark
    Pages 1-20
  3. Kelly M. Fulton, Susan M. Twine
    Pages 21-57
  4. Ugur Sahin, Özlem Türeci
    Pages 59-77
  5. Elisa Beghetto, Nicola Gargano
    Pages 79-95
  6. Kelly M. Fulton, Shannon S. Martin, Lawrence Wolfraim, Susan M. Twine
    Pages 97-112
  7. N. Helena Beyer, Niels H. H. Heegaard
    Pages 123-140
  8. Luc Tessier, Kelly M. Fulton, Susan M. Twine
    Pages 181-195
  9. Richard Ouedraogo, Julien Textoris, Aurélie Daumas, Christian Capo, Jean-Louis Mege
    Pages 197-209
  10. Richard H. Maier, Christina J. Maier, Kamil Önder
    Pages 211-229
  11. Timothy J. Waybright, King C. Chan, Timothy D. Veenstra, Zhen Xiao
    Pages 279-289
  12. Theodoros A. Koutroukides, Julian A. J. Jaros, Bob Amess, Daniel Martins-de-Souza, Paul C. Guest, Hassan Rahmoune et al.
    Pages 291-307
  13. Scott M. Ferguson
    Pages 361-367
  14. Back Matter
    Pages 369-370

About this book


Providing current diverse approaches and techniques used to study the immunoproteome, Immunoproteomics: Methods and Protocols collects chapters from key researchers that deliver information to be used in diagnostics, disease progression, and vaccine correlates of protection analysis, to name but a few. This detailed volume includes techniques used for the study of the antibody targets of bacterial pathogens, viruses, and cancer, mass spectrometry-based approaches to characterize T-cell epitopes, chapters on detection and relative quantification of cytokines in serum, as well as in silico prediction of epitopes using sequence-based or modeling approaches.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and thorough, Immunoproteomics: Methods and Protocols aids researchers in transferring these techniques to their own laboratories in addition to providing a reference to guide researchers toward appropriate techniques.


Adaptive immune system Antibody targets Bacterial pathogens Cancer Microbial antigen discovery The immunoproteome Viruses

Editors and affiliations

  • Kelly M. Fulton
    • 1
  • Susan M. Twine
    • 2
  1. 1.National Research Council Canada Human Health TherapeuticsOttawaCanada
  2. 2.National Research Council Canada Human Health TherapeuticsOttawaCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-588-0
  • Online ISBN 978-1-62703-589-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site