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Neural Development

Methods and Protocols

  • Book
  • © 2013


  • Includes clear and current techniques for the study of neural development
  • Provides step-by-step detail essential for reproducible results
  • Contains key tips and expert implementation advice to ensure success in the lab

Part of the book series: Methods in Molecular Biology (MIMB, volume 1018)

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About this book

Understanding the molecular and cellular mechanisms underlying the development of specific neural circuits is not just an intellectual curiosity but also central to our ability to develop therapeutic approaches to repair damaged pathways in the future. In Neural Development: Methods and Protocols, experts in the field contribute commonly used protocols to facilitate future research in developmental neuroscience. Split into four convenient sections, this detailed volume covers techniques of culturing neurons and glia as well as their growth and differentiation, methods of gene delivery and down regulation, protocols for analyzing axon growth and guidance plus synapse formation, and, finally, basic methods to analyze brain morphology and axon pathways in developing animals.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and accessible, Neural Development: Methods and Protocols provides key guidance for students and postdoctoral fellows new to developmental neurobiology.

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Table of contents (31 protocols)

  1. Neuron and Glial Cell Culture

  2. Gene Expression and Analysis

Editors and Affiliations

  • , Department of Chemical Biology, Rutgers University, Piscataway, USA

    Renping Zhou

  • , Inst. Molecular Medicine & Genetics, Georgia Health Sciences University, Augusta, USA

    Lin Mei

Bibliographic Information

  • Book Title: Neural Development

  • Book Subtitle: Methods and Protocols

  • Editors: Renping Zhou, Lin Mei

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-443-2Published: 17 May 2013

  • Softcover ISBN: 978-1-4939-6018-7Published: 23 August 2016

  • eBook ISBN: 978-1-62703-444-9Published: 17 May 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 347

  • Number of Illustrations: 11 b/w illustrations, 48 illustrations in colour

  • Topics: Neurosciences, Neurobiology, Cell Culture

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