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Chemokines

Methods and Protocols

  • Astrid E. Cardona
  • Eroboghene E. Ubogu

Part of the Methods in Molecular Biology book series (MIMB, volume 1013)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Sandra M. Cardona, Jenny A. Garcia, Astrid E. Cardona
    Pages 1-16
  3. Luigi Buonaguro, Maria Tagliamonte, Maria Luisa Visciano
    Pages 57-66
  4. Alexandre Garin, Zoë Johnson, Aurelie Hermant, Fanny Beltran, Yann Ratinaud, Alexandra Michel et al.
    Pages 67-92
  5. Irina Kufareva, Bryan Stephens, C. Taylor Gilliland, Beili Wu, Gustavo Fenalti, Damon Hamel et al.
    Pages 93-127
  6. Simon Yona, Ki-Wook Kim, Rebecca Haffner, Steffen Jung
    Pages 129-144
  7. Eva Kochhan, Arndt F. Siekmann
    Pages 145-159
  8. Anne-Marie Baird, Kenneth J. O’Byrne, Steven G. Gray
    Pages 185-201
  9. Laura B. Ford, Chris A. H. Hansell, Robert J. B. Nibbs
    Pages 203-214
  10. Back Matter
    Pages 235-238

About this book

Introduction

Chemokines constitute a large family of structurally similar cytokines that contain a signature of conserved cysteine residues joined by disulfide bridges.  Binding of chemokines to specific G protein-coupled receptors followed by downstream signaling defines their biological function.  Initially, chemoattraction was the key function linked to chemokines/chemokine receptors; however, in recent years, it has become clear that chemokine ligand-receptor interactions can also modulate cellular activation, survival, and proliferation, among other functions in homeostatic and diseased states.  Importantly, major advances in our understanding of chemokine biology have led to chemokine receptors becoming specific therapeutic targets with great potential.  In Chemokines: Methods and Protocols, expert researchers provide practical information regarding experimental models and state of the art protocols used to delineate chemokine/chemokine receptor function and their applications in health and disease.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and easy to use, Chemokines: Methods and Protocols aims to reveal key protocols of functional and descriptive chemokine ligand/receptor assays that will be of practical significance to graduate students, post-doctoral fellows, trainees, and researchers in academia and industry.

Keywords

Chemokine function Chemokine ligand G protein-coupled receptors Therapeutic targets

Editors and affiliations

  • Astrid E. Cardona
    • 1
  • Eroboghene E. Ubogu
    • 2
  1. 1.Department of Biology, South Texas Center for Emerging Infectious DiseasesUniversity of Texas at San AntonioSan AntonioUSA
  2. 2., Department of NeurologyBaylor College of MedicineHoustonUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-426-5
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-425-8
  • Online ISBN 978-1-62703-426-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site