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Vascular Proteomics

Methods and Protocols

  • Fernando Vivanco

Part of the Methods in Molecular Biology book series (MIMB, volume 1000)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Maria G. Barderas, Fernando Vivanco, Gloria Alvarez-Llamas
    Pages 1-20
  3. Fernando de la Cuesta, Gloria Alvarez-Llamas, Aroa S. Maroto, Maria G. Barderas, Fernando Vivanco
    Pages 21-32
  4. Sebastián Mas, Raúl Pérez, Jesús Egido
    Pages 33-43
  5. Jianhua Zhang, Jiahua Shi, Benedict Jia Hong Lee, Liwei Chen, Kee Yang Tan, Xiaoling Tang et al.
    Pages 45-52
  6. Alejandro Zimman, Judith A. Berliner, Thomas G. Graeber
    Pages 53-69
  7. Gloria Alvarez-Llamas, Fernando de la Cuesta, Maria G. Barderas, Irene Zubiri, Maria Posada-Ayala, Fernando Vivanco
    Pages 71-80
  8. Fernando de la Cuesta, Maria G. Barderas, Enrique Calvo, Irene Zubiri, Aroa S. Maroto, Juan Antonio Lopez et al.
    Pages 81-90
  9. Roxana Martinez-Pinna, Juan Antonio Lopez, Priscila Ramos-Mozo, Luis M. Blanco-Colio, Emilio Camafeita, Enrique Calvo et al.
    Pages 91-101
  10. Michal Ciborowski, Coral Barbas
    Pages 103-113
  11. Elena Burillo, Jesus Vazquez, Inmaculada Jorge
    Pages 139-156
  12. Enrique Calvo, Roxana Martínez-Pinna, Priscila Ramos-Mozo, C. Pastor-Vargas, Emilio Camafeita, Jesús Egido et al.
    Pages 157-166
  13. Andrew J. Percy, Andrew G. Chambers, Carol E. Parker, Christoph H. Borchers
    Pages 167-189
  14. Verónica M. Dardé, Maria G. Barderas, Fernando Vivanco
    Pages 191-199
  15. Back Matter
    Pages 221-230

About this book

Introduction

Proteomics is a rapidly expanding investigation platform in cardiovascular medicine. Driven by major improvements in mass spectrometry (MS) instrumentation and data analysis, the proteomics field has flourished in recent years particularly in the study of complex diseases. These recent advances are characterized by the development of quantitative MS-based methods that promoted the field from primarily identifying proteins to also providing measurements of relative changes in protein levels between different cell states. This progress is reflected in the application of proteomic techniques to vascular pathology. Vascular Proteomics: Methods and Protocols provides up-to-date methods and protocols for the analysis of arteries, cells, lipoproteins, body fluids, and metabolites, with a particular focus on MS-based methods of protein and peptide quantification. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Vascular Proteomics: Methods and Protocols is a representative selection of methods that can be a useful resource for experienced proteomics practitioners as well as newcomers interested in becoming acquainted with the practice of proteomic techniques for cardiovascular research.

Keywords

aortic cells cardiovascular medicine mass spectrometry proteomics vascular biology

Editors and affiliations

  • Fernando Vivanco
    • 1
  1. 1.Depto. ImmunologíaFundación Jiménez DíazMadridSpain

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-405-0
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-404-3
  • Online ISBN 978-1-62703-405-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site