Skip to main content
  • Book
  • © 2013

Cytochrome P450 Protocols

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB)

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, access via your institution.

Table of contents (25 protocols)

  1. Front Matter

    Pages i-xii
  2. Bioluminescent Assays for Cytochrome P450 Enzymes

    • Douglas S. Auld, Henrike Veith, James J. Cali
    Pages 1-9
  3. High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening

    • Kheng B. Lim, Can C. Ozbal, Daniel B. Kassel
    Pages 25-50
  4. The Synthesis, Characterization, and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites

    • Christopher McCullough, Phani Kumar Pullela, Sang-Choul Im, Lucy Waskell, Daniel Sem
    Pages 51-59
  5. High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators

    • Cesar Kenaan, Haoming Zhang, Paul F. Hollenberg
    Pages 61-69
  6. Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium tuberculosis

    • Hugues Ouellet, Eric D. Chow, Shenheng Guan, Jeffery S. Cox, Alma L. Burlingame, Paul R. Ortiz de Montellano
    Pages 79-94
  7. An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme Proteins

    • Michael B. Winter, Joshua J. Woodward, Michael A. Marletta
    Pages 95-106
  8. Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group

    • Wesley E. Straub, Clinton R. Nishida, Paul R. Ortiz de Montellano
    Pages 107-113
  9. Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 Enzymes

    • A. Luthra, M. Gregory, Y. V. Grinkova, I. G. Denisov, S. G. Sligar
    Pages 115-127
  10. Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles

    • Linlin Zhao, Besnik Bajrami, James F. Rusling
    Pages 129-134
  11. Fluorescence-Based Screening of Cytochrome P450 Activities in Intact Cells

    • M. Teresa Donato, M. José Gómez-Lechón
    Pages 135-148
  12. Screening for Cytochrome P450 Reactivity with a Reporter Enzyme

    • Kersten S. Rabe, Christof M. Niemeyer
    Pages 149-156
  13. High-Throughput Fluorescence Assay of Cytochrome P450 3A4

    • Qian Cheng, F. Peter Guengerich
    Pages 157-162
  14. Targeted Protein Capture for Analysis of Electrophile-Protein Adducts

    • Rebecca E. Connor, Simona G. Codreanu, Lawrence J. Marnett, Daniel C. Liebler
    Pages 163-176
  15. DNA Shuffling of Cytochrome P450 Enzymes

    • James B. Y. H. Behrendorff, Wayne A. Johnston, Elizabeth M. J. Gillam
    Pages 177-188
  16. Measurement of P450 Difference Spectra Using Intact Cells

    • Wayne A. Johnston, Elizabeth M. J. Gillam
    Pages 189-204

About this book

Cytochromes P450 (CYPs) comprise a large superfamily of proteins that are of central importance in the detoxification or activation of a tremendous number of natural and synthetic hydrophobic xenobiotics, including many therapeutic drugs, chemical carcinogens and environmental pollutants. CYPs are important in mediating interactions between an organism and its chemical environment and in the regulation of physiological processes. Cytochrome P450 Protocols, Third Edition focuses on high-throughput methods for the simultaneous analysis of multiple CYPs, substrates or ligands. Although the emphasis is on CYPs of mammalian origin, it reflects an increasing interest in CYPs of bacterial species. Also included are chapters on cytochrome P450 reductase (the redox partner of CYPs) and the flavin-containing monooxygenases (FMOs), and metabolomic and lipidomic approaches for identification of endogenous substrates of CYPs (‘de-orphanizing’ CYP substrates). Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Cytochrome P450 Protocols, Third Edition provides a wide range of techniques accessible to researchers in fields as diverse as biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics.

Keywords

  • CYP enzymes
  • Cytochrome P450 enzymes
  • DNA shuffling
  • LC-MS
  • mass spectrometry

Reviews

From the reviews of the third edition:

“With this third edition, the authors and contributors continue to provide what has become a tour de force of bench top manuals for the cytochrome P450 field. … this book should also prove of interest to graduate students and postdoctoral researchers who are new to the field of P450 science, or who would like to expand their research directions. … This stands alone as the premier book for this field, which is sorely lacking in publications of this type.” (Jed N. Lampe, Doody’s Book Reviews, October, 2013)

Editors and Affiliations

  • Sch. of Biological & Chemical Sciences, Queen Mary, University of London, London, United Kingdom

    Ian R. Phillips

  • , Department of Structural and Molecular B, University College London, London, United Kingdom

    Elizabeth A. Shephard

  • University of California, San Francisco, USA

    Paul R. Ortiz de Montellano

Bibliographic Information

  • Book Title: Cytochrome P450 Protocols

  • Editors: Ian R. Phillips, Elizabeth A. Shephard, Paul R. Ortiz de Montellano

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-321-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-320-6Published: 09 March 2013

  • Softcover ISBN: 978-1-4939-6269-3Published: 23 August 2016

  • eBook ISBN: 978-1-62703-321-3Published: 12 March 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XII, 319

  • Topics: Protein Science, Protein-Ligand Interactions

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access