Skip to main content
  • Book
  • © 2013

Gene Regulation

Methods and Protocols

Editors:

  • Comprehensive and broad in their scope, the protocols are useful to researchers in many disciplines

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 977)

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-1-62703-284-1
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 179.00
Price excludes VAT (USA)
Hardcover Book USD 179.00
Price excludes VAT (USA)

This is a preview of subscription content, access via your institution.

Table of contents (31 protocols)

  1. Front Matter

    Pages i-xv
  2. Gene Regulation

    • Minou Bina
    Pages 1-11
  3. Isolation and Analysis of DNA Derived from Nucleosome-Free Regions

    • Matthew Murtha, Yatong Wang, Claudio Basilico, Lisa Dailey
    Pages 35-51
  4. Acquisition of High Quality DNA for Massive Parallel Sequencing by In Vivo Chromatin Immunoprecipitation

    • M. van den Boogaard, L. Y. E. Wong, V. M. Christoffels, P. Barnett
    Pages 53-64
  5. Promoter Deletion Analysis Using a Dual-Luciferase Reporter System

    • Yong Zhong Xu, Cynthia Kanagaratham, Sylwia Jancik, Danuta Radzioch
    Pages 79-93
  6. Identifying Specific Protein–DNA Interactions Using SILAC-Based Quantitative Proteomics

    • Cornelia G. Spruijt, H. Irem Baymaz, Michiel Vermeulen
    Pages 137-157
  7. Using FRET to Monitor Protein-Induced DNA Bending: The TBP-TATA Complex as a Model System

    • Rebecca H. Blair, James A. Goodrich, Jennifer F. Kugel
    Pages 203-215

About this book

In this volume of Methods in Molecular Biology™, expert investigators offer comprehensive, complementary, and cutting-edge technologies for studies of gene regulation.  The chapters of Gene Regulation: Methods and Protocols are organized to provide an integrated and a coherent view of control systems and their associated components.  The protocols are broad in their scope.  They include molecular, biochemical, spectroscopic techniques as well as high throughput strategies.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Comprehensive and broad in their scope, the protocols are useful to researchers in many disciplines including molecular biology, genomics, biochemistry, biomedicine, nutrition, and agricultural sciences.    

FEATURES
• Techniques for studying chromatin structural features that influence gene activation
In vivo and in vitro assays
• Spectroscopic techniques
• Technologies for examining genome-wide association of transcription factors with regulatory regions of genes
• Proteomics based on SILAC (Stable Isotope Labeling of Amino Acids in Cell Culture)
• Peptide microarray technology for studies of kinases
• Schemes for isolation and characterization of relatively large multiprotein complexes
• Biochemical and biophysical techniques for examining gene repression
In vitro chromatin reconstitution to investigate multicomponent transcriptional complexes
• Nuclear recruitment assay to validate transcription factor interactions in mammalian cells

Keywords

  • DNA sequencing
  • DNase hypersensitivity analysis
  • Heavy methyl-SILAC
  • de novo identification
  • gene activation
  • gene repression
  • histone methylation patterns
  • histone modifications
  • mass spectrometry (LC-MS)
  • protein networks
  • protein-DNA interactions
  • quantitative liquid chromatography
  • gene expression

Editors and Affiliations

  • , Department of Chemistry, Purdue University, West Lafayette, USA

    Minou Bina

Bibliographic Information

  • Book Title: Gene Regulation

  • Book Subtitle: Methods and Protocols

  • Editors: Minou Bina

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-284-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-283-4

  • Softcover ISBN: 978-1-4939-6288-4

  • eBook ISBN: 978-1-62703-284-1

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 401

  • Number of Illustrations: 61 b/w illustrations, 19 illustrations in colour

  • Topics: Medical Genetics, Molecular Genetics

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-1-62703-284-1
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 179.00
Price excludes VAT (USA)
Hardcover Book USD 179.00
Price excludes VAT (USA)