© 2012

Recombinant and In Vitro RNA Synthesis

Methods and Protocols

  • Graeme L. Conn
  • Details a collection of protocols that guide the reader from the start of a new RNA project to the final sample

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 941)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Luc Ponchon, Geneviève Beauvais, Sylvie Nonin-Lecomte, Frédéric Dardel
    Pages 9-18
  3. Jessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy, Loren Dean Williams
    Pages 19-41
  4. Cheng Lu, Pingwei Li
    Pages 59-68
  5. Evan P. Booy, Hui Meng, Sean A. McKenna
    Pages 69-81
  6. Milena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski, Jerzy Ciesiolka
    Pages 99-111
  7. Hae-Kap Cheong, Eunha Hwang, Chaejoon Cheong
    Pages 113-121
  8. Geneviève Di Tomasso, Philipe Lampron, James G. Omichinski, Pascale Legault
    Pages 123-135
  9. Geneviève Di Tomasso, Pierre Dagenais, Alexandre Desjardins, Alexis Rompré-Brodeur, Vanessa Delfosse, Pascale Legault
    Pages 137-155
  10. Stephanie L. Moon, Jeffrey Wilusz
    Pages 171-180
  11. N. Ruth Zearfoss, Sean P. Ryder
    Pages 181-193
  12. Lina Lin, Zhen Huang
    Pages 213-225
  13. Christopher J. Kershaw, Raymond T. O’Keefe
    Pages 257-269

About this book


The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule. In Recombinant and In Vitro RNA Synthesis: Methods and Protocols, expert researchers in the field detail approaches from the inception of a new RNA project to the final sample ready for analysis. These experimental protocols combine established work-horse approaches with some of the latest innovative methods for RNA synthesis and purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Recombinant and In Vitro RNA Synthesis: Methods and Protocols aids scientists in continuing to study the fascinating world of RNA.


RNA purification RNA synthesis biochemical biophysical chemical synthesis gel filtration chromatography in vitro transcription recombinant approach recursive PCR protocol ribozymes

Editors and affiliations

  • Graeme L. Conn
    • 1
  1. 1.School of Medicine, Dept. BiochemistryEmory UniversityAtlantaUSA

Bibliographic information

  • Book Title Recombinant and In Vitro RNA Synthesis
  • Book Subtitle Methods and Protocols
  • Editors Graeme L. Conn
  • Series Title Methods in Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-62703-112-7
  • Softcover ISBN 978-1-4939-5830-6
  • eBook ISBN 978-1-62703-113-4
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIV, 274
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Human Genetics
    Nucleic Acid Chemistry
  • Buy this book on publisher's site