© 2013

Biolistic DNA Delivery

Methods and Protocols

  • Stephan Sudowe
  • Angelika B. Reske-Kunz

Part of the Methods in Molecular Biology book series (MIMB, volume 940)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Biolistic DNA Delivery in Plant Science and Agricultural Biotechnology

    1. Front Matter
      Pages 1-1
    2. Shoko Ueki, Shimpei Magori, Benoît Lacroix, Vitaly Citovsky
      Pages 17-26
    3. Mulpuri Sujatha, K. B. R. S. Visarada
      Pages 27-44
    4. Anssi L. Vuorinen, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, Jari P. T. Valkonen
      Pages 45-51
    5. Kevin M. Davies, Simon C. Deroles, Murray R. Boase, Don A. Hunter, Kathy E. Schwinn
      Pages 63-74
  3. Biolistic DNA Delivery in Nematodes

    1. Front Matter
      Pages 75-75
    2. Meltem Isik, Eugene Berezikov
      Pages 77-86
    3. Annabel A. Ferguson, Liquan Cai, Luv Kashyap, Alfred L. Fisher
      Pages 87-102
    4. Tarig B. Higazi, Thomas R. Unnasch
      Pages 103-115
  4. Biolistic DNA Delivery in Mammalian Cells In Vitro

    1. Front Matter
      Pages 117-117
    2. Xiongwei Li, Masaki Uchida, H. Oya Alpar, Peter Mertens
      Pages 119-132
    3. Kandan Aravindaram, Shu-Yi Yin, Ning-Sun Yang
      Pages 133-143
    4. David F. Steele, Ying Dou, David Fedida
      Pages 145-155
    5. John A. O’Brien, Sarah C. R. Lummis
      Pages 157-166
  5. Biolistic DNA Delivery in Mammalian Cells In Vivo

    1. Front Matter
      Pages 167-167
    2. Meng-Chi Yen, Ming-Derg Lai
      Pages 169-174

About this book


Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun.  Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


All-inclusive and cutting-edge,  Biolistic DNA Delivery: Methods and Protocols brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field.


Artificial DNA introduction Biolistic transfection DNA vaccination Gene guns Gene transfer

Editors and affiliations

  • Stephan Sudowe
    • 1
  • Angelika B. Reske-Kunz
    • 2
  1. 1.Ganzimmun Diagnostics AGMainzGermany
  2. 2.University Medical Center, Department of DermatologyJohannes Gutenberg-UniversityMainzGermany

Bibliographic information

  • Book Title Biolistic DNA Delivery
  • Book Subtitle Methods and Protocols
  • Editors Stephan Sudowe
    Angelika B. Reske-Kunz
  • Series Title Methods in Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-62703-109-7
  • Softcover ISBN 978-1-4939-6068-2
  • eBook ISBN 978-1-62703-110-3
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIV, 415
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Human Genetics
    Genetic Engineering
  • Buy this book on publisher's site