Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 938)
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About this book
Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply. Phytoplasmas can cause serious and devastating problems for crop plants, especially in developing countries. In Phytoplasmas: Methods and Protocols experts in the field cover topics pertinent to scientists intending to develop a research program on phytoplasmas. The volume opens with an introductory chapter on the importance of phytoplasma diseases, followed by methods for detection and diagnosis as well as techniques for separating and classifying the phytoplasmas into their different taxonomic groups and subgroups. The final chapters cover methods for separating phytoplasma genomic and plasmid DNA from plant DNA for whole genome sequencing. Written in the successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and easily accessible, Phytoplasmas: Methods and Protocols serves as an ideal guide to plant pathologists and molecular biologists aiming to set up a diagnostics facility for identifying the presence of these pathogens in plants or their insect vectors.
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Keywords
Table of contents (34 protocols)
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Front Matter
Editors and Affiliations
Bibliographic Information
Book Title: Phytoplasma
Book Subtitle: Methods and Protocols
Editors: Matt Dickinson, Jennifer Hodgetts
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-089-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-088-5Published: 18 September 2012
Softcover ISBN: 978-1-4939-5965-5Published: 23 August 2016
eBook ISBN: 978-1-62703-089-2Published: 18 September 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 421