Overview
- Fully updates and expands upon the successful miRNA methodologies of the first edition
- Features potential applications in cosmetics, wound healing, and prostate cancer
- Includes key tips and implementation advice to ensure successful results
Part of the book series: Methods in Molecular Biology (MIMB, volume 936)
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Table of contents (27 protocols)
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Back Matter
Keywords
About this book
Extensive studies have been conducted on the identification, biogenesis, and processing of microRNA (miRNA) as well as research on the exact mechanism by which miRNAs bring about translational silencing of their targets. In addition, numerous publications point to an important role of miRNAs in development, reprogramming, epigenetics, pathogenesis of cancer, oncogenes and tumor suppressors, biomarkers of various disease onset, and regulation of adipogenesis and obesity; yet many questions still remain. In MicroRNA Protocols, Second Edition, experts in the field provide up-to-date coverage of this diverse area of study. The specific chapters of this edition are related to the analysis of miRNA, targets and expression profiling, various methods to determine its regulation of gene expression, the preparation and isolation of miRNAs in specific tissues, its detection in the saliva, and potential application in cosmetics, wound healing, and prostate cancer. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and known pitfalls.
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Fully updated and easy to use, MicroRNA Protocols, Second Edition aims to stimulate readers to explore diverse ways to understand the mechanisms in which miRNAs facilitate the molecular aspects of not only biomedical research but also a wide range of other research fields.Editors and Affiliations
Bibliographic Information
Book Title: MicroRNA Protocols
Editors: Shao-Yao Ying
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-083-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-082-3Published: 26 September 2012
Softcover ISBN: 978-1-4939-6261-7Published: 23 August 2016
eBook ISBN: 978-1-62703-083-0Published: 25 September 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XI, 375
Topics: Human Genetics, Gene Expression