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  • © 2012

Odontogenesis

Methods and Protocols

Editors:

  • Serves as a guide to basic and state-of-the-art techniques to understand anatomical and molecular dental phenotypes

  • Provides cutting-edge, step-by-step protocols, ready for the lab

  • Features tips from the experts on how to successfully complete the methods

Part of the book series: Methods in Molecular Biology (MIMB, volume 887)

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eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-61779-860-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 149.00
Price excludes VAT (USA)
Hardcover Book USD 199.99
Price excludes VAT (USA)

This is a preview of subscription content, access via your institution.

Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
  2. Determination of Gene Expression Patterns by Whole-Mount In Situ Hybridization

    • Sergiy Kyryachenko, Kateryna Kyrylkova, Mark Leid, Chrissa Kioussi
    Pages 15-22
  3. Determination of Gene Expression Patterns by In Situ Hybridization in Sections

    • Kateryna Kyrylkova, Sergiy Kyryachenko, Chrissa Kioussi, Mark Leid
    Pages 23-31
  4. Immunohistochemistry and Detection of Proliferating Cells by BrdU

    • Sergiy Kyryachenko, Kateryna Kyrylkova, Mark Leid, Chrissa Kioussi
    Pages 33-39
  5. Detection of Apoptosis by TUNEL Assay

    • Kateryna Kyrylkova, Sergiy Kyryachenko, Mark Leid, Chrissa Kioussi
    Pages 41-47
  6. Use of siRNA in Dental Tissue-Derived Cell Cultures: Integrin Knockdown in Fibroblasts

    • Malgorzata M. Barczyk, Donald Gullberg, Anne Isine Bolstad
    Pages 49-57
  7. Organ Cultures and Kidney-Capsule Grafting of Tooth Germs

    • Keishi Otsu, Naoki Fujiwara, Hidemitsu Harada
    Pages 59-67
  8. Electron Microscopy

    • Hans U. Luder, Margrit Amstad-Jossi
    Pages 81-93
  9. Deoxyoligonucleotide Microarrays for Gene Expression Profiling in Murine Tooth Germs

    • Harald Osmundsen, Anne-Marthe Jevnaker, Maria A. Landin
    Pages 95-110
  10. Lineage Differentiation of Mesenchymal Stem Cells from Dental Pulp, Apical Papilla, and Periodontal Ligament

    • Kentaro Akiyama, Chider Chen, Stan Gronthos, Songtao Shi
    Pages 111-121
  11. In Vivo Transplantation and Tooth Repair

    • Shuhei Tsuchiya, Masaki J. Honda
    Pages 123-134
  12. Methods to Validate Tooth-Supporting Regenerative Therapies

    • Miguel Padial-Molina, Julie T. Marchesan, Andrei D. Taut, Qiming Jin, William V. Giannobile, Hector F. Rios
    Pages 135-148
  13. Generation of a Bioengineered Tooth by Using a Three-Dimensional Cell Manipulation Method (Organ Germ Method)

    • Masamitsu Oshima, Miho Ogawa, Masato Yasukawa, Takashi Tsuji
    Pages 149-165
  14. In Vitro Studies on Odontogenic Tumors

    • Javier Catón, Thimios A. Mitsiadis, Peter R. Morgan
    Pages 167-177
  15. Back Matter

    Pages 193-194

About this book

Only in recent times has the possibility of growing and implanting replacement teeth, made from one’s own cells, moved into the realm of realistic possibilities; however, the molecular and cellular mechanisms of tooth development must be studied in a range of vertebrates, from zebrafish to mice, so that evolutionarily conserved network kernels, which will define the cellular states of generic vertebrate tooth development, can be recognized.  In Odontogenesis: Methods and Protocols, experts in the field examine techniques to approach this burgeoning field.  This detailed volume includes chapters on the detection of “tooth development” gene expression, both at the RNA and protein level, current approaches to the manipulation of gene expression levels and subsequent analysis of tooth phenotypes, as well as chapters concerning current efforts to get living tooth implants working without waiting for a full understanding of the developmental pathways at the molecular level.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for trouble-shooting and avoiding known pitfalls.

 

Practical and easy to use, Odontogenesis: Methods and Protocols aims to help researchers move forward toward the ultimate goal of getting a “bioengineered tooth” into the patient’s mouth.

Keywords

  • Bioengineered teeth
  • Developmental mechanism
  • Gene perturbation
  • Living tooth implants
  • Odontogenesis
  • Tooth development
  • Tooth repair
  • gene expression

Editors and Affiliations

  • College of Pharmacy, Dept. of Pharmaceutical Sciences, Oregon State University, Corvallis, USA

    Chrissa Kioussi

Bibliographic Information

  • Book Title: Odontogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Chrissa Kioussi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-860-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-859-7

  • Softcover ISBN: 978-1-4939-5947-1

  • eBook ISBN: 978-1-61779-860-3

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 194

  • Number of Illustrations: 16 b/w illustrations, 24 illustrations in colour

  • Topics: Cell Biology, Developmental Biology and Stem Cells, Molecular Genetics

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-61779-860-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 149.00
Price excludes VAT (USA)
Hardcover Book USD 199.99
Price excludes VAT (USA)