Overview
- Details methodolgies that can be modified and applied to the studies of rential development
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 884)
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About this book
In recent years, there have been major advances in the concepts and methodologies used in the study of retinal development at both cellular and molecular levels. These advanced methodologies have allowed and will continue to allow researchers to gain new insights into the molecular mechanisms underlying retinal development. In Retinal Development: Methods and Protocols, expert researchers in the field detail many of the protocols used for a wide range of experiments. These include protocols and techniques for manipulating gene expression in vivo, tracing cell fates with modernized classic blastomere manipulation in Xenopus and with Cre-based technique in mouse and in zebrafish, retinal regeneration and stem cell-based replacement, and ERG (function) recording and non-invasive imaging. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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Authoritative and practical, Retinal Development: Methods and Protocols provides methodologies crucial to the success of increasingly more complex and often challenging investigations in the fields of retinal development and other biological and biomedical research.Â
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Keywords
Table of contents (25 protocols)
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Manipulation of Gene Expression in vivo
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Tracing Cell Fate
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In vitro Systems
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Regeneration/Stem Cells
Editors and Affiliations
Bibliographic Information
Book Title: Retinal Development
Book Subtitle: Methods and Protocols
Editors: Shu-Zhen Wang
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-848-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-847-4Published: 12 June 2012
Softcover ISBN: 978-1-4939-6252-5Published: 04 May 2017
eBook ISBN: 978-1-61779-848-1Published: 12 June 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 367
Number of Illustrations: 58 b/w illustrations, 21 illustrations in colour
Topics: Molecular Medicine, Developmental Biology