Somatic Stem Cells

Methods and Protocols

  • Shree Ram Singh

Part of the Methods in Molecular Biology book series (MIMB, volume 879)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Introduction

    1. Front Matter
      Pages 1-1
  3. Stem Cell Study in Lower Organism

    1. Front Matter
      Pages 27-27
    2. Tetsutaro Hayashi, Kiyokazu Agata
      Pages 29-37
    3. Mo Weng, Hideyuki Komori, Cheng-Yu Lee
      Pages 39-46
    4. Shree Ram Singh, Manoj K. Mishra, Madhuri Kango-Singh, Steven X. Hou
      Pages 47-69
  4. Stem Cell Study in Murine and Human Model

    1. Front Matter
      Pages 71-71
    2. Barbara Driscoll, Alex Kikuchi, Allison N. Lau, Jooeun Lee, Raghava Reddy, Edwin Jesudason et al.
      Pages 109-122
    3. Kristbjorn Orri Gudmundsson, Steven W. Stull, Jonathan R. Keller
      Pages 123-133
    4. Laurent Lescaudron, C. Boyer, Virginie Bonnamain, K. D. Fink, X. Lévêque, J. Rossignol et al.
      Pages 147-164
    5. Sofia Grade, Fabienne Agasse, Liliana Bernardino, João O. Malva
      Pages 165-178
    6. Abhik Bandyopadhyay, Qiaoxiang Dong, Lu-Zhe Sun
      Pages 179-193
    7. Steven J. Greco, Shyam A. Patel, Pranela Rameshwar
      Pages 195-201

About this book


 Stem cells are found in almost all organisms from the early stages of development to the end of life. There are several types of stem cells and all of them may prove useful for medical research; however, each of the different types has both promise and limitations. Somatic Stem Cells: Methods and Protocols presents selected genetic, molecular, and cellular techniques used in somatic stem cell research and its clinical application. Chapters focus on the isolation, characterization, purity, plasticity, and clinical uses of somatic stem cells from a variety of human and animal tissues. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Through and intuitive, Somatic Stem Cells: Methods and Protocols seeks to provides scientists with the fundamental techniques of stem cell research and its potential application in regenerative medicine.


Somatic Stem cells anticancer therapies embryonic stem cells pluripotent stem cells regenerative medicine stem cell differentiation stem cell proliferation tissue homeostasis

Editors and affiliations

  • Shree Ram Singh
    • 1
  1. 1.Mouse Cancer Genetics ProgramNational Cancer InstituteFrederickUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-814-6
  • Online ISBN 978-1-61779-815-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book