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  • Book
  • © 2012

Plant Signalling Networks

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 876)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
  2. Experimental Analysis of Receptor Kinase Phosphorylation

    • Srijeet K. Mitra, Michael B. Goshe, Steven D. Clouse
    Pages 1-15
  3. Identification of O-linked β-d-N-acetylglucosamine-Modified Proteins from Arabidopsis

    • Shou-Ling Xu, Robert J. Chalkley, Zhi-Yong Wang, Alma L. Burlingame
    Pages 33-45
  4. Small-Molecule Dissection of Brassinosteroid Signaling

    • Mirela-Corina Codreanu, Dominique Audenaert, Long Nguyen, Tom Beeckman, Eugenia Russinova
    Pages 95-106
  5. Activation Tagging

    • Xiaoping Gou, Jia Li
    Pages 117-133
  6. In Vivo Ubiquitination Assay by Agroinfiltration

    • Lijing Liu, Qingzhen Zhao, Qi Xie
    Pages 153-162
  7. In Vitro Protein Ubiquitination Assay

    • Qingzhen Zhao, Lijing Liu, Qi Xie
    Pages 163-172
  8. Smart Pooling of mRNA Samples for Efficient Transcript Profiling

    • Raghunandan M. Kainkaryam, Angela Bruex, Peter J. Woolf, John Schiefelbein
    Pages 189-194
  9. Genome-Wide Profiling of Uncapped mRNA

    • Yuling Jiao, José Luis Riechmann
    Pages 207-216
  10. Computational Tools for Quantitative Analysis of Cell Growth Patterns and Morphogenesis in Actively Developing Plant Stem Cell Niches

    • Anirban Chakraborty, Ram Kishor Yadav, Min Liu, Moses Tataw, Katya Mkrtchyan, Amit Roy Chowdhury et al.
    Pages 217-227
  11. Back Matter

    Pages 229-230

About this book

Signal transduction is the fundamental mechanism for regulation of cellular activities by environmental cues and regulatory signals, and is particularly important for plants, whose survival requires proper physiological and developmental responses to the environmental changes. Much progress has been made recently in the plant signal transduction research field thanks to the development of diverse techniques which are covered in Plant Signalling Networks: Methods and Protocols. These include advanced research methods such as proteomics and mass spectrometry methods for studying protein modification, biochemical and cell biological tools for studying protein-protein interactions, genomic techniques for dissecting protein-DNA interaction and transcription networks, and computation methods that integrate molecular network into plant developmental processes. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Plant Signalling Networks: Methods and Protocols presents well-honed methodologies for a wide range of research approaches including genetics, proteomics, biochemical, cell biological, and computational approaches, and seeks to serve both professionals and novices with a comprehensive understanding of complex signaling networks in plants. 

Reviews

From the reviews:

“This protocol book is a good overview across various research approaches such as genomics, proteomics, metabolomics, and bioinformatics. Its methods are elaborate and clear, and most of the chapters provide specific hints and notes to increase the success of the experiments. This book will long stand as a guide to plant signaling networks, and it can be taken as a useful vademecum to the lab for the more experienced user.” (Peter Schröder, Plant Science Bulletin, Vol. 59 (1), 2013)

Editors and Affiliations

  • Dept. Plant Biology, Carnegie Institution, Stanford, USA

    Zhi-Yong Wang

  • Dept. Botany & Plant Science, University of California, Riverside, Riverside, USA

    Zhenbiao Yang

Bibliographic Information

  • Book Title: Plant Signalling Networks

  • Book Subtitle: Methods and Protocols

  • Editors: Zhi-Yong Wang, Zhenbiao Yang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-809-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-808-5Published: 14 May 2012

  • Softcover ISBN: 978-1-4939-6169-6Published: 23 August 2016

  • eBook ISBN: 978-1-61779-809-2Published: 11 May 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 230

  • Number of Illustrations: 21 b/w illustrations, 7 illustrations in colour

  • Topics: Plant Sciences, Proteomics

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access