Overview
- Defines gene synthesis and determines what part of the process labs can do and what parts can be contracted to a specialized service provider
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 852)
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Table of contents (21 protocols)
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Synthon Assembly
Keywords
About this book
The de novo fabrication of custom DNA molecules is a transformative technology that significantly affects the biotechnology industry. Basic genetic engineering techniques for manipulating DNA in vitro opened an incredible field of opportunity in the life sciences. In, Gene Synthesis: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to fabricate DNA . These include methods and techniques for the assembly of oligonucleotide, cloning of synthons into larger fragments, protocols and software applications, and educational and biosecurity impacts of gene synthesis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Thorough and intuitive, Gene Synthese: Methods and Protocols aids scientists in understanding all the different stages of a complex gene synthesis process, while refining their understanding of gene synthesis and determine what part of the process they can or should do in their laboratory and what parts should be contracted to a specialized service provider.
Editors and Affiliations
Bibliographic Information
Book Title: Gene Synthesis
Book Subtitle: Methods and Protocols
Editors: Jean Peccoud
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-564-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Sceince+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-563-3Published: 10 February 2012
Softcover ISBN: 978-1-4939-6200-6Published: 23 August 2016
eBook ISBN: 978-1-61779-564-0Published: 11 February 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 297
Number of Illustrations: 35 b/w illustrations, 42 illustrations in colour
Topics: Human Genetics, Genetic Engineering