Controlled Genetic Manipulations

  • Alexei Morozov

Part of the Neuromethods book series (NM, volume 65)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Kimi Araki, Ken-ichi Yamamura
    Pages 29-45
  3. Anke Van der Perren, Jaan Toelen, Jean-Marc Taymans, Veerle Baekelandt
    Pages 47-68
  4. Gregory A. Dissen, Jodi McBride, Alejandro Lomniczi, Valerie Matagne, Mauricio Dorfman, Tanaya L. Neff et al.
    Pages 69-96
  5. Jan Christoph Koch, Mathias Bähr, Paul Lingor
    Pages 97-111
  6. Leah R. DeBlander, Aldis P. Weible, Clifford G. Kentros
    Pages 165-191
  7. Kazuto Kobayashi, Kana Okada, Nobuyuki Kai
    Pages 193-205
  8. Feng Zhang, Le Cong, Garret D. Stuber, Antoine Adamantidis, Karl Deisseroth
    Pages 207-223
  9. Back Matter
    Pages 225-227

About this book


The current demand for the development of techniques for controlled genetic manipulations is driven by the anatomical and physiological complexity of the brain and by the need for experimental models that can address this complexity through selective manipulation of defined components of the system: specific neuronal populations or selected synapses.  In Controlled Genetic Manipulations, expert researchers present detailed chapters to supply basic technical information about controlled genetic manipulations and to provide examples of creative implementation of this methodology when addressing a unique biological problem. Some chapters of the book describe the most recent developments in the basic methodology, which includes use of Cre-recombinase, methods for delivery of genetic material into the brain and the use of optogenetics, whereas other chapters focus on applying these techniques to addressing particular biological questions like structural and functional mapping of neuronal circuits, analysis of specific synaptic connections, modeling or gene therapy of neurological disorders.  As part of the Neuromethods series, chapters include key implementation advice that is crucial for getting optimal results.


Authoritative and practical, Controlled Genetic Manipulations serves as a valuable guide to a variety of techniques, provided by many of the pioneers of the approaches.


Animal models Behavioral models Cre-recombinase Genetic manipulations Mouse models Neuronal circuits Neurophysiology Optogenetics Synaptic connections

Editors and affiliations

  • Alexei Morozov
    • 1
  1. 1.National Institute of Mental HealthNational Institutes of HealthBethesdaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-532-9
  • Online ISBN 978-1-61779-533-6
  • Series Print ISSN 0893-2336
  • Series Online ISSN 1940-6045
  • Buy this book on publisher's site