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  • © 2012

Ancient DNA

Methods and Protocols

Editors:

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  • Closes the gap between appropriate methodologicol approaches and retrieval and anysis of ancient DNA

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 840)

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eBook
USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-61779-516-9
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 139.99
Price excludes VAT (USA)
Hardcover Book
USD 199.99
Price excludes VAT (USA)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
  2. Setting Up an Ancient DNA Laboratory

    • Tara L. Fulton
    Pages 1-11
  3. Case Study: Recovery of Ancient Nuclear DNA from Toe Pads of the Extinct Passenger Pigeon

    • Tara L. Fulton, Stephen M. Wagner, Beth Shapiro
    Pages 29-35
  4. Extraction of DNA from Paleofeces

    • Melanie Kuch, Hendrik Poinar
    Pages 37-42
  5. DNA Extraction from Keratin and Chitin

    • Paula F. Campos, Thomas M. P. Gilbert
    Pages 43-49
  6. Case Study: Ancient Sloth DNA Recovered from Hairs Preserved in Paleofeces

    • Andrew A. Clack, Ross D. E. MacPhee, Hendrik N. Poinar
    Pages 51-56
  7. DNA Extraction from Fossil Eggshell

    • Charlotte L. Oskam, Michael Bunce
    Pages 65-70
  8. Ancient DNA Extraction from Plants

    • Logan Kistler
    Pages 71-79
  9. DNA Extraction from Formalin-Fixed Material

    • Paula F. Campos, Thomas M. P. Gilbert
    Pages 81-85
  10. PCR Amplification, Cloning, and Sequencing of Ancient DNA

    • Tara L. Fulton, Mathias Stiller
    Pages 111-119
  11. Quantitative Real-Time PCR in aDNA Research

    • Michael Bunce, Charlotte L. Oskam, Morten E. Allentoft
    Pages 121-132
  12. Multiplex PCR Amplification of Ancient DNA

    • Mathias Stiller, Tara L. Fulton
    Pages 133-141
  13. Preparation of Next-Generation Sequencing Libraries from Damaged DNA

    • Adrian W. Briggs, Patricia Heyn
    Pages 143-154
  14. Generating Barcoded Libraries for Multiplex High-Throughput Sequencing

    • Michael Knapp, Mathias Stiller, Matthias Meyer
    Pages 155-170

About this book

Research into ancient DNA began more than 25 years ago with the publication of short mitochondrial DNA sequence fragments from the quagga, an extinct relative of the zebra. Ancient DNA research really gained momentum following the invention of PCR, which allowed millions of copies to be made of the few remaining DNA molecules preserved in fossils and museum specimens.  In Ancient DNA: Methods and Protocols expert researchers in the field describe many of the protocols that are now commonly used to study ancient DNA. These include instructions for setting up an ancient DNA laboratory, extraction protocols for a wide range of different substrates, details of laboratory techniques including PCR and NGS library preparation, and suggestions for appropriate analytical approaches to make sense of the sequences obtained. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Ancient DNA: Methods and Protocols seeks to aid scientists in the further study of ancient DNA and the methodological approaches in ancient research.

Keywords

  • NGS
  • PCR
  • Pleistocene-age DNA
  • ancient DNA
  • ancient bone
  • bone hydroxyl-apatite
  • hydroxyl-apatite crystals
  • mitochondrial genomes
  • phylogeographic patterns
  • soft tissue

Editors and Affiliations

  • Dept. of Ecology and Evolutionary Biolog, University of California Santa Cruz, Santa Cruz, USA

    Beth Shapiro

  • , Department of Biology (Area 2), University of York, York, United Kingdom

    Michael Hofreiter

Bibliographic Information

  • Book Title: Ancient DNA

  • Book Subtitle: Methods and Protocols

  • Editors: Beth Shapiro, Michael Hofreiter

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-516-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-515-2

  • Softcover ISBN: 978-1-4939-5874-0

  • eBook ISBN: 978-1-61779-516-9

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 247

  • Number of Illustrations: 14 b/w illustrations, 12 illustrations in colour

  • Topics: Medical Genetics

Buying options

eBook
USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-61779-516-9
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 139.99
Price excludes VAT (USA)
Hardcover Book
USD 199.99
Price excludes VAT (USA)