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Plant Fungal Pathogens

Methods and Protocols

  • Book
  • © 2012


  • Highlights the latest techniques for the study of molecular plant pathology
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes an implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 835)

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Table of contents (41 protocols)


About this book

Over the course of evolution, fungi have adapted to occupy specific niches, from symbiotically inhabiting the flora of the intestinal tract of mammals to saprophytic growth on leaf litter resting on the forest floor.  In Plant Fungal Pathogens: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study fungal plant pathogens. These include methods and techniques for model systems such as Arabidopsis thaliana as well as crop plants, aspects of fungal biology, genome annotation, next-generation sequencing, and fungal transformation and molecular tools for disease and/or pathogen quantification that are critical for revealing the role for a fungal gene of interest in disease development. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Plant Fungal Pathogens: Methods and Protocols seeks to aid scientists in the further study in current techniques that cover a wide-range of methods to study molecular aspects of pathogenesis.


From the reviews:

“The authors provide the latest information on topics including methods in molecular biology and highlighting some of the latest techniques critical for students pursuing molecular plant pathology. … All the chapters are well illustrated with charts and graphs and with necessary photographs. … should be available in school libraries experienced fungal research laboratories and university libraries. This is specially aimed at researchers new to the molecular biology field.” (Asha Janadaree Dissanayake and Kevin D. Hyde, Fungal Diversity, Vol. 59, March, 2013)

“The current volume is a unique collection of 41 chapters from a diverse group of scientists, researchers, and academics working on different aspects of the molecular basis of fungal pathogens and pathogenesis. … This volume will be useful for both students and researchers in the fields of botany and forestry, agriculture, mycology, microbiology, plant disease and diagnostics, molecular plant pathology, horticulture science, biomolecular sciences, life sciences, fungal molecular biology, fungal biotechnology, microbial biotechnology, and food technology.” (S. K. Basu, Plant Science Bulletin, Vol. 59 (1), 2013)

Editors and Affiliations

  • Northern Crops Science Laboratory, USDA-ARS, Fargo, USA

    Melvin D. Bolton

  • Lab. Phytopathology, Wageningen University, Wageningen, Netherlands

    Bart P.H.J. Thomma

Bibliographic Information

  • Book Title: Plant Fungal Pathogens

  • Book Subtitle: Methods and Protocols

  • Editors: Melvin D. Bolton, Bart P.H.J. Thomma

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-500-8Published: 21 December 2011

  • Softcover ISBN: 978-1-4939-5898-6Published: 23 August 2016

  • eBook ISBN: 978-1-61779-501-5Published: 21 December 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 648

  • Topics: Plant Sciences, Plant Pathology

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