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Amino Acid Analysis

Methods and Protocols

  • Book
  • © 2012


  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material:

Part of the book series: Methods in Molecular Biology (MIMB, volume 828)

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Table of contents (28 protocols)


About this book

Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs. In this volume, a reader can find chapters describing general as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry as well as in food analysis, microbiology, marine biology, drug metabolism, even archeology.  Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Amino Acid Analysis: Methods and Protocols provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.

Editors and Affiliations

  • Ctr. Biologics Evaluation & Research, Div. Cellular & Gene Therapies, U.S. Food and Drug Administration, Bethesda, USA

    Michail A. Alterman

  • Functional Genomics Center Zürich, Protein Analysis Group, Universität Zürich, Zürich, Switzerland

    Peter Hunziker

Bibliographic Information

  • Book Title: Amino Acid Analysis

  • Book Subtitle: Methods and Protocols

  • Editors: Michail A. Alterman, Peter Hunziker

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-444-5Published: 02 December 2011

  • Softcover ISBN: 978-1-4939-5889-4Published: 23 August 2016

  • eBook ISBN: 978-1-61779-445-2Published: 02 February 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 363

  • Topics: Protein Science, Analytical Chemistry

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