Rho GTPases

Methods and Protocols

  • Francisco Rivero

Part of the Methods in Molecular Biology book series (MIMB, volume 827)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. History and Phylogenesis

    1. Front Matter
      Pages 1-1
    2. Anne J. Ridley
      Pages 3-12
  3. Biochemistry of Rho GTPases

    1. Front Matter
      Pages 35-35
    2. Mamta Jaiswal, Badri N. Dubey, Katja T. Koessmeier, Lothar Gremer, Mohammad R. Ahmadian
      Pages 37-58
    3. Heike Bielek, Gudula Schmidt
      Pages 59-75
    4. Anne Doye, Amel Mettouchi, Emmanuel Lemichez
      Pages 77-86
    5. Natalia Mitin, Patrick J. Roberts, Emily J. Chenette, Channing J. Der
      Pages 87-95
  4. Functional Assays

    1. Front Matter
      Pages 121-121
    2. Nicolas Reymond, Philippe Riou, Anne J. Ridley
      Pages 123-142
    3. Sébastien Nola, Jennifer C. Erasmus, Vania M. M. Braga
      Pages 143-155
    4. Esben Pedersen, Astrid Basse, Tine Lefever, Karine Peyrollier, Cord Brakebusch
      Pages 157-166
    5. Roland Leung, Michael Glogauer
      Pages 167-179
    6. Daniel Feltrin, Olivier Pertz
      Pages 181-194
  5. Imaging and High Throughput Methods

    1. Front Matter
      Pages 213-213
    2. Désirée Spiering, Louis Hodgson
      Pages 215-234

About this book

Introduction

Although initially described as major regulators of cytoskeletal remodeling, Rho GTPases have been implicated in the establishment of polarity, endocytosis, vesicle trafficking, morphogenesis, cytokinesis, transcriptional activation, cell cycle progression, and apoptosis, to mention a few. In addition, Rho GTPases have acquired medical relevance because of their participation in tumorigenesis and metastasis, in cardiovascular conditions, and as targets of infectious agents. The field has broadened even more with the contribution of studies in model organisms (plants, amoebas, fungi, invertebrates), each adding a particular view to the complexity of the family and vastly enriching our perception of these important signaling components. Divided into five convenient sections, Rho GTPase: Methods and Protocols provides an historical overview of the field and an account of the phylogenetics of the Rho family, general biochemical methods, and functional assays that allow monitoring the consequences of manipulating Rho GTPases in a variety of contexts. Additionally, the volume devotes a section to advanced imaging methods and to recently developed high throughput methods, closing with techniques specifically designed for studies in selected non-mammalian model organisms. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Rho GTPase: Methods and Protocols provides techniques that are standard for researchers in the field but also includes protocols for those who, being already familiar with some of the techniques, wish to explore additional aspects.

Keywords

Cdc42 Rac Rho GDI Rho GTPases signaling complex protein family

Editors and affiliations

  • Francisco Rivero
    • 1
  1. 1.Hull York Medical School, Centre for Biomedical ResearchUniversity of HullHullUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-442-1
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-441-4
  • Online ISBN 978-1-61779-442-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book