© 2012


Methods and Protocols

  • Thomas Weichhart

Part of the Methods in Molecular Biology book series (MIMB, volume 821)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Sungki Hong, Aristotle M. Mannan, Ken Inoki
    Pages 29-44
  3. Douangsone D. Vadysirisack, Leif W. Ellisen
    Pages 45-58
  4. Dos D. Sarbassov, Olga Bulgakova, Rakhmet I. Bersimbaev, Tattym Shaiken
    Pages 59-74
  5. Mahmoud Khalil, Ivan Gout
    Pages 87-103
  6. Margit Rosner, Markus Hengstschläger
    Pages 105-124
  7. Lorenzo Galluzzi, Eugenia Morselli, Oliver Kepp, Ilio Vitale, Aména Ben Younes, Maria Chiara Maiuri et al.
    Pages 125-169
  8. Valentina Iadevaia, Xuemin Wang, Zhong Yao, Leonard J. Foster, Christopher G. Proud
    Pages 171-185
  9. Angela Papageorgiou, Joseph Avruch
    Pages 187-214
  10. Jinhee Kim, Nancy Otto, Claudio J. Conti, Irma B. Gimenz-Conti, Cheryl L. Walker
    Pages 215-225
  11. Beat Nyfeler, Philip Bergman, Christopher J. Wilson, Leon O. Murphy
    Pages 239-250
  12. Nadine Cybulski, Vittoria Zinzalla, Michael N. Hall
    Pages 267-278
  13. Manuela Battaglia, Angela Stabilini, Eleonora Tresoldi
    Pages 279-293
  14. Chinnaswamy Jagannath, Pearl Bakhru
    Pages 295-303

About this book


Since its discovery, the mammalian target of rapamycin (mTOR) has been shown to regulate many critical molecular processes in eukaryotes such as metabolism, growth, survival, aging, synaptic plasticity, memory, and immunity. In mTOR: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study mTOR. These include methods and techniques  used for the study of the mTOR pathway and potential therapeutic applications of mTOR inhibitors  such as, immunosuppressive and anticancer agents. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, mTOR: Methods and Protocols seeks to aid scientists in the further study of this crucially important molecule  ranging from the analysis of signal transduction events within a cell to the assessment of complex human diseases such as metabolic disorders or cancer.


cancer research energy metabolism immunology mTOR mammalian target of rapamycin metabolic disorders molecular cell biology transplantation

Editors and affiliations

  • Thomas Weichhart
    • 1
  1. 1.Division of Nephrology and DialysisMedical University of ViennaViennaAustria

Bibliographic information

  • Book Title mTOR
  • Book Subtitle Methods and Protocols
  • Editors Thomas Weichhart
  • Series Title Methods in Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-61779-429-2
  • Softcover ISBN 978-1-4939-6209-9
  • eBook ISBN 978-1-61779-430-8
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIII, 444
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Cell Biology
  • Buy this book on publisher's site