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Functional Genomics

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Michael Kaufmann

    1. School of Medicine, Witten/Herdecke University, Witten, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Claudia Klinger

    1. School of Medicine, Private Universität Witten/Herdecke, Witten, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Useful for all scientists who plan to establish or extend one of the technologies described in the book in their own labs.
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 815)

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Table of contents (29 protocols)

  1. Protein analysis I: quantification and identification

    1. Mass Spectrometry for Protein Quantification in Biomarker Discovery

      • Mu Wang, Jinsam You
      Pages 199-225

  2. Protein Analysis II: Functional Characterization

    1. Front Matter

      Pages 227-227
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  3. Protein analysis II: functional characterization

    1. High-Throughput Microtitre Plate-Based Assay for DNA Topoisomerases

      • James A. Taylor, Nicolas P. Burton, Anthony Maxwell
      Pages 229-239

    2. Microscale Thermophoresis as a Sensitive Method to Quantify Protein: Nucleic Acid Interactions in Solution

      • Karina Zillner, Moran Jerabek-Willemsen, Stefan Duhr, Dieter Braun, Gernot Längst, Philipp Baaske
      Pages 241-252

    3. Bioluminescence Resonance Energy Transfer: An Emerging Tool for the Detection of Protein–Protein Interaction in Living Cells

      • Søren W. Gersting, Amelie S. Lotz-Havla, Ania C. Muntau
      Pages 253-263

    4. LuMPIS: Luciferase-Based MBP-Pull-Down Protein Interaction Screening System

      • María G. Vizoso Pinto, Armin Baiker
      Pages 265-275

    5. Yeast Two-Hybrid Screens: Improvement of Array-Based Screening Results by N- and C-terminally Tagged Fusion Proteins

      • Thorsten Stellberger, Roman Häuser, Peter Uetz, Albrecht von Brunn
      Pages 277-288

    6. Inducible microRNA-Mediated Knockdown of the Endogenous Human Lamin A/C Gene

      • Ina Weidenfeld
      Pages 289-305

    7. Multiple-Gene Silencing Using Antisense RNAs in Escherichia coli

      • Nobutaka Nakashima, Shan Goh, Liam Good, Tomohiro Tamura
      Pages 307-319

    8. Functional Screen of Zebrafish Deubiquitylating Enzymes by Morpholino Knockdown and In Situ Hybridization

      • William Ka Fai Tse, Yun-Jin Jiang
      Pages 321-331

    9. Silencing of Gene Expression by Gymnotic Delivery of Antisense Oligonucleotides

      • Harris S. Soifer, Troels Koch, Johnathan Lai, Bo Hansen, Anja Hoeg, Henrik Oerum et al.
      Pages 333-346

    10. Polycistronic Expression of Interfering RNAs from RNA Polymerase III Promoters

      • Laura F. Steel, Viraj R. Sanghvi
      Pages 347-359

  4. Metabolite Analysis

    1. Front Matter

      Pages 361-361
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  5. Metabolite analysis

    1. Metabolite Analysis of Cannabis sativa L. by NMR Spectroscopy

      • Isvett Josefina Flores-Sanchez, Young Hae Choi, Robert Verpoorte
      Pages 363-375

    2. Metabolome Analysis of Gram-Positive Bacteria such as Staphylococcus aureus by GC-MS and LC-MS

      • Manuel Liebeke, Kirsten Dörries, Hanna Meyer, Michael Lalk
      Pages 377-398

    3. Metabolic Fingerprinting Using Comprehensive Two-Dimensional Gas Chromatography – Time-of-Flight Mass Spectrometry

      • Martin F. Almstetter, Peter J. Oefner, Katja Dettmer
      Pages 399-411

  6. Back Matter

    Pages 413-418
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Keywords

About this book

Over the last decade Life Science has undergone an accelerated evolution, culminating in the -omics era characterized by the development of a multitude of high throughput methods that are becoming more routinely applied in biochemistry labs. In Functional Genomics: Methods and Protocols, Second Edition expert researchers in the field detail many of the methods which are now commonly used for studies in the life sciences focusing on the dynamic aspects of the transcriptome, proteome and metabolome, respectively.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Functional Genomics: Methods and Protocols, Second Edition seeks to aid scientists in establishing  or extending technologies and techniques in their laboratories.

Editors and Affiliations

  • School of Medicine, Witten/Herdecke University, Witten, Germany

    Michael Kaufmann

  • School of Medicine, Private Universität Witten/Herdecke, Witten, Germany

    Claudia Klinger

Bibliographic Information

  • Book Title: Functional Genomics

  • Book Subtitle: Methods and Protocols

  • Editors: Michael Kaufmann, Claudia Klinger

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-424-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-423-0Published: 02 December 2011

  • Softcover ISBN: 978-1-4939-6228-0Published: 23 August 2016

  • eBook ISBN: 978-1-61779-424-7Published: 02 December 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 418

  • Topics: Human Genetics

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