© 2012

Bone Research Protocols

  • Miep H. Helfrich
  • Stuart H. Ralston


  • Contains a catalogue of protocols to assist researchers in the pursuit of mechanistic studies

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 816)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Culture of Osteoblasts and Osteocytes

    1. Front Matter
      Pages 1-1
    2. Jane P. Dillon, Victoria J. Waring-Green, Adam M. Taylor, Peter J. M. Wilson, Mark Birch, Alison Gartland et al.
      Pages 3-18
    3. Astrid D. Bakker, Jenneke Klein-Nulend
      Pages 19-29
    4. Isabel R. Orriss, Sarah E. B. Taylor, Timothy R. Arnett
      Pages 31-41
    5. Cor M. Semeins, Astrid D. Bakker, Jenneke Klein-Nulend
      Pages 43-53
    6. Christine Halleux, Ina Kramer, Cyril Allard, Michaela Kneissel
      Pages 55-66
    7. Jennifer Rosser, Lynda F. Bonewald
      Pages 67-81
    8. Rahul S. Tare, Peter D. Mitchell, Janos Kanczler, Richard O. C. Oreffo
      Pages 83-99
  3. Culture of Osteoclasts

    1. Front Matter
      Pages 101-101
    2. Isabel R. Orriss, Timothy R. Arnett
      Pages 103-117
    3. Patricia Collin-Osdoby, Philip Osdoby
      Pages 119-143
    4. Fraser P. Coxon, Michael J. Rogers, Julie C. Crockett
      Pages 145-158
    5. Kim Henriksen, Morten A. Karsdal, Adam Taylor, Denise Tosh, Fraser P. Coxon
      Pages 159-175
    6. Cecile Itzstein, Robert J. van ‘t Hof
      Pages 177-186
    7. Patricia Collin-Osdoby, Philip Osdoby
      Pages 187-202
  4. Biochemical and Molecular Analysis of Bone Cells

    1. Front Matter
      Pages 203-203
    2. Julie C. Crockett, David J. Mellis, Adam Taylor
      Pages 205-222
    3. Huilin Jin, Stuart H. Ralston
      Pages 233-247

About this book


Studies over the past decade have continued to bring tremendous advances to our understanding of bone biology. New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength. In Bone Research Protocols, Second Edition, expert researchers in the field detail many methods commonly used to study bone biology. Focusing mainly on in vitro methods, this volume gives techniques for isolation, culture and functional analysis of all bone cell types and details a range of imaging methods, including light and ultrastructural microscopy and live cell imaging. Some important in vivo techniques are included, such as analysis of bone resorption and imaging using X rays, fluorescent or luminescent techniques. Methods for study of proteins and nucleic acid are included and methods for analysis of bone composition, measurement of bone strength, and response to mechanical stimulation are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Bone Research Protocols, Second Edition seeks to aid scientists in the bone field to establish new techniques in their laboratories.


Bone Research biochemical analysis bone mass bone strength in vivo loading techniques inherited bone disorders mesenchymal stem cells molecular analysis osteoblast cultures

Editors and affiliations

  • Miep H. Helfrich
    • 1
  • Stuart H. Ralston
    • 2
  1. 1.Institute of Medical SciencesUniversity of AberdeenAberdeenUnited Kingdom
  2. 2.Institute of Genetics and Molecular Medi, Rheumatic Diseases UnitUniversity of EdinburghEdinburghUnited Kingdom

Bibliographic information

  • Book Title Bone Research Protocols
  • Editors Miep H. Helfrich
    Stuart H. Ralston
  • Series Title Methods in Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-61779-414-8
  • Softcover ISBN 978-1-4939-6100-9
  • eBook ISBN 978-1-61779-415-5
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 2
  • Number of Pages XIV, 607
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Cell Biology
    Developmental Biology
    Animal Anatomy / Morphology / Histology
  • Buy this book on publisher's site


From the reviews of the second edition:

“This volume presents detailed modern experimental methods to study bone biology. For bone researchers.” (Pediatric Endocrinology Reviews (PER), Vol. 9 (3), March, 2012)