Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 813)
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Table of contents (23 protocols)
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Front Matter
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Design Strategies for Synthetic Gene Network
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Front Matter
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Development and Characterization of the Synthetic Biology Toolbox
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Front Matter
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Optimizing the Host Systems
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Front Matter
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Keywords
About this book
The rapid expansion of synthetic biology is due to the design and construction of synthetic gene networks that have opened many new avenues in fundamental and applied research. Synthetic Gene Networks: Methods and Protocols provides the necessary information to design and construct synthetic gene networks in different host backgrounds. Divided into four convenient sections, this volume focuses on design concepts to devise synthetic gene networks and how mathematical models can be applied to the predictable engineering of desired network features. The volume continues by highlighting the construction and validation of biologic tools, describing strategies to optimize and streamline the host cell for optimized network performance, and covering how optimally designed gene networks can be implemented in a large variety of host cells ranging from bacteria over yeast and insect cells to plant and mammalian cell culture. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Synthetic Gene Networks: Methods and Protocols serves as an invaluable resource for established biologists, engineers, and computer scientists or novices just entering into the rapidly growing field of synthetic biology
Editors and Affiliations
Bibliographic Information
Book Title: Synthetic Gene Networks
Book Subtitle: Methods and Protocols
Editors: Wilfried Weber, Martin Fussenegger
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-412-4
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-411-7Published: 15 November 2011
Softcover ISBN: 978-1-4939-6224-2Published: 23 August 2016
eBook ISBN: 978-1-61779-412-4Published: 16 November 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 393
Number of Illustrations: 64 b/w illustrations, 3 illustrations in colour
Topics: Bioinformatics, Systems Biology