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  • © 2012

Allostery

Methods and Protocols

Editors:

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  • Contains expert protocols, the common feature of which are allosteric systems, i.e. ligand-induced, through-protein changes

  • Draws together the study of protein structure/function and the study of allostery

  • Features key tips and implementation advice to ensure successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 796)

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USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-1-61779-334-9
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 179.00
Price excludes VAT (USA)
Hardcover Book
USD 179.00
Price excludes VAT (USA)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xviii
  2. Monitoring Allosteric Function

    1. Front Matter

      Pages 1-1
    2. Binding Techniques to Study the Allosteric Energy Cycle

      • James K. Kranz, José C. Clemente
      Pages 3-17
    3. Kinetic Trapping of a Key Hemoglobin Intermediate

      • Jo M. Holt, Gary K. Ackers
      Pages 19-29
    4. Studying the Allosteric Energy Cycle by Isothermal Titration Calorimetry

      • Marta Martinez-Julvez, Olga Abian, Sonia Vega, Milagros Medina, Adrian Velazquez-Campoy
      Pages 53-70
    5. Detecting “Silent” Allosteric Coupling

      • Harvey F. Fisher
      Pages 71-96
    6. Using Mutant Cycle Analysis to Elucidate Long-Range Functional Coupling in Allosteric Receptors

      • Jai A. P. Shanata, Shawnalea J. Frazier, Henry A. Lester, Dennis A. Dougherty
      Pages 97-113
  3. Monitoring Allosteric Conformational Changes

    1. Front Matter

      Pages 115-115
    2. Allosteric Mechanisms of G Protein-Coupled Receptor Signaling: A Structural Perspective

      • Tarjani M. Thaker, Ali I. Kaya, Anita M. Preininger, Heidi E. Hamm, T. M. Iverson
      Pages 133-174
    3. Dynamic Light Scattering to Study Allosteric Regulation

      • Aaron L. Lucius, P. Keith Veronese, Ryan P. Stafford
      Pages 175-186
    4. Fluorescence Correlation Spectroscopy and Allostery: The Case of GroEL

      • Gabriel A. Frank, Amnon Horovitz, Gilad Haran
      Pages 205-216
  4. Monitoring Allosteric Changes in Protein Dynamics/Subpopulation Distribution

    1. Front Matter

      Pages 233-233
  5. Monitoring Allosteric Changes in Protein Dynamics/Sub-Population Distribution

    1. Ensemble Properties of Network Rigidity Reveal Allosteric Mechanisms

      • Donald J. Jacobs, Dennis R. Livesay, James M. Mottonen, Oleg K. Vorov, Andrei Y. Istomin, Deeptak Verma
      Pages 279-304
  6. Macromolecular and Ligand Engineering Allosteric Functions

    1. Front Matter

      Pages 305-305

About this book

Despite considerable variability within the scientific community, allosteric regulation can best be defined functionally as how a macromolecule binds one ligand differently when a second ligand is or is not pre-bound to the macromolecule, which constitutes a vital aspect of protein structure/function.  In Allostery: Methods and Protocols, expert researchers in the field provide key techniques to investigate this biological phenomenon.  Focusing on heterotropic systems with some coverage of homotropic systems, this volume covers the monitoring of allosteric function, allosteric conformational changes, and allosteric changes in protein dynamics/sub-population distribution, as well as topics such as macromolecular and ligand engineering of allosteric functions and computational aids in the study of allostery. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

Thorough and intuitive, Allostery: Methods and Protocols aids scientists in continuing to study ligand-induced, through-protein effects on protein function (ligand binding/catalysis), a phenomenon that is well recognized through the history of the life sciences and very poorly understood at the molecular level.

Keywords

  • Allosteric regulation
  • Computational methods
  • Conformational changes
  • Cooperative
  • Heterotropic
  • Homotropic
  • Ligands
  • Protein dynamics
  • Protein structure/function
  • Sub-population distribution
  • Protein Structure

Editors and Affiliations

  • , Dept. Biochemistry & Molecular Biology, University of Kansas Medical Center, Kansas City, USA

    Aron W. Fenton

Bibliographic Information

  • Book Title: Allostery

  • Book Subtitle: Methods and Protocols

  • Editors: Aron W. Fenton

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-334-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-333-2

  • Softcover ISBN: 978-1-4939-5864-1

  • eBook ISBN: 978-1-61779-334-9

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 439

  • Number of Illustrations: 134 b/w illustrations

  • Topics: Protein Biochemistry, Proteins

Buying options

eBook
USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-1-61779-334-9
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 179.00
Price excludes VAT (USA)
Hardcover Book
USD 179.00
Price excludes VAT (USA)