Editors:
- Contains expert protocols, the common feature of which are allosteric systems, i.e. ligand-induced, through-protein changes
- Draws together the study of protein structure/function and the study of allostery
- Features key tips and implementation advice to ensure successful results
Part of the book series: Methods in Molecular Biology (MIMB, volume 796)
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Table of contents (23 protocols)
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Front Matter
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Monitoring Allosteric Function
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Front Matter
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Monitoring Allosteric Conformational Changes
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Front Matter
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Monitoring Allosteric Changes in Protein Dynamics/Subpopulation Distribution
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Front Matter
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Monitoring Allosteric Changes in Protein Dynamics/Sub-Population Distribution
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Macromolecular and Ligand Engineering Allosteric Functions
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Front Matter
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About this book
Despite considerable variability within the scientific community, allosteric regulation can best be defined functionally as how a macromolecule binds one ligand differently when a second ligand is or is not pre-bound to the macromolecule, which constitutes a vital aspect of protein structure/function. In Allostery: Methods and Protocols, expert researchers in the field provide key techniques to investigate this biological phenomenon. Focusing on heterotropic systems with some coverage of homotropic systems, this volume covers the monitoring of allosteric function, allosteric conformational changes, and allosteric changes in protein dynamics/sub-population distribution, as well as topics such as macromolecular and ligand engineering of allosteric functions and computational aids in the study of allostery. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Thorough and intuitive, Allostery: Methods and Protocols aids scientists in continuing to study ligand-induced, through-protein effects on protein function (ligand binding/catalysis), a phenomenon that is well recognized through the history of the life sciences and very poorly understood at the molecular level.
Editors and Affiliations
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, Dept. Biochemistry & Molecular Biology, University of Kansas Medical Center, Kansas City, USA
Aron W. Fenton
Bibliographic Information
Book Title: Allostery
Book Subtitle: Methods and Protocols
Editors: Aron W. Fenton
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-334-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-333-2Published: 04 November 2011
Softcover ISBN: 978-1-4939-5864-1Published: 23 August 2016
eBook ISBN: 978-1-61779-334-9Published: 02 December 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVIII, 439
Number of Illustrations: 134 b/w illustrations
Topics: Protein Science, Protein Structure