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  • © 2011

Chloroplast Research in Arabidopsis

Methods and Protocols, Volume II

Editors:

  • Brings together the most important and current techniques in chloroplast research through the unifying lens of the Arabidopsis model system
  • Features subjects like protein aspects, omics studies, and photosynthesis and biochemical analysis
  • Includes expert tips and implementation advice to guarantee ideal experimental results

Part of the book series: Methods in Molecular Biology (MIMB, volume 775)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
  2. Omics and Large-Scale Analyses

    1. Front Matter

      Pages 91-91
    2. Proteome Databases and Other Online Resources for Chloroplast Research in Arabidopsis

      • Diogo Ribeiro Demartini, Célia Regina Carlini, Jay J. Thelen
      Pages 93-115
    3. Use of Non-aqueous Fractionation and Metabolomics to Study Chloroplast Function in Arabidopsis

      • Peter Geigenberger, Axel Tiessen, Jörg Meurer
      Pages 135-160
  3. Proteomics and Suborganellar Fractionation

    1. Front Matter

      Pages 187-187
    2. Preparation of Envelope Membrane Fractions from Arabidopsis Chloroplasts for Proteomic Analysis and Other Studies

      • Daniel Salvi, Lucas Moyet, Daphné Seigneurin-Berny, Myriam Ferro, Jacques Joyard, Norbert Rolland
      Pages 189-206
    3. Preparation of Plastoglobules from Arabidopsis Plastids for Proteomic Analysis and Other Studies

      • Celine Besagni, Lucia Eugeni Piller, Claire Bréhélin
      Pages 223-239
  4. Photosynthesis and Biochemical Analysis

    1. Front Matter

      Pages 297-297

About this book

Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable.  The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid.  In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume II explores topics such as multiprotein complexes, protein-protein interactions, omics and large-scale analyses, proteomics and suborganellar fractionation, as well as photosynthesis and biochemical analysis.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.

Editors and Affiliations

  • , Department of Biology, University of Leicester, Leicester, United Kingdom

    R. Paul Jarvis

Bibliographic Information

  • Book Title: Chloroplast Research in Arabidopsis

  • Book Subtitle: Methods and Protocols, Volume II

  • Editors: R. Paul Jarvis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-237-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-236-6Published: 23 August 2011

  • Softcover ISBN: 978-1-4939-6289-1Published: 23 August 2016

  • eBook ISBN: 978-1-61779-237-3Published: 23 August 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 432

  • Number of Illustrations: 56 b/w illustrations, 5 illustrations in colour

  • Topics: Plant Sciences, Plant Biochemistry

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access