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Molecular Methods for Evolutionary Genetics

  • Book
  • © 2011

Overview

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 772)

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About this book

We are entering a particularly fruitful period in evolutionary genetics, as rapid technological progress transforms the investigation of genetic variation within and between species. Molecular Methods for Evolutionary Genetics is a collection of advanced molecular biology protocols and general overviews intended to represent the essential methods currently bringing evolutionary genetics to fruition. Divided into six thematic sections, this volume covers methods for characterizing genomes, diverse approaches to enrich DNA for subsets of the genome prior to sequencing, and state-of-the-art protocols for sampling genetic variation for genetic mapping studies and population genetic studies (RAD sequencing, Sequenom, microarrays, etc.). The volume concludes by focusing on methods to study candidate genes, from obtaining their sequences and analyzing their transcripts to experimentally manipulating their activities in vivo. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Molecular Methods for Evolutionary Genetics serves as a rich resource to biologists interested in evolution, whether they be specialists or beginners in molecular biology.

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Keywords

Table of contents (28 protocols)

  1. Characterizing the Genome

  2. Targeting Regions of the Genome

  3. Measuring Genetic Diversity

  4. Obtaining Candidate Gene Sequences

Editors and Affiliations

  • CNRS UMR7592, Université Paris VII, Paris, France

    Virginie Orgogozo

  • Dept. Biology, New York University, New York, USA

    Matthew V. Rockman

Bibliographic Information

  • Book Title: Molecular Methods for Evolutionary Genetics

  • Editors: Virginie Orgogozo, Matthew V. Rockman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-228-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-227-4Published: 29 September 2011

  • Softcover ISBN: 978-1-4939-5861-0Published: 23 August 2016

  • eBook ISBN: 978-1-61779-228-1Published: 09 November 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 503

  • Number of Illustrations: 77 b/w illustrations, 4 illustrations in colour

  • Topics: Human Genetics, Gene Function

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