Molecular Methods for Evolutionary Genetics

  • Virginie Orgogozo
  • Matthew V. Rockman

Part of the Methods in Molecular Biology book series (MIMB, volume 772)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Characterizing the Genome

    1. Front Matter
      Pages 1-1
    2. David M. Rowell, Shu Ly Lim, Frank Grutzner
      Pages 13-35
    3. Mike A. Quail, Lucy Matthews, Sarah Sims, Christine Lloyd, Helen Beasley, Simon W. Baxter
      Pages 37-58
    4. Mike A. Quail, Lucy Matthews, Sarah Sims, Christine Lloyd, Helen Beasley, Simon W. Baxter
      Pages 59-81
  3. Targeting Regions of the Genome

    1. Front Matter
      Pages 83-83
    2. Heiko Vogel, Christopher W. Wheat
      Pages 105-128
    3. Christopher W. Wheat, Heiko Vogel
      Pages 129-144
  4. Measuring Genetic Diversity

    1. Front Matter
      Pages 155-155
    2. Paul D. Etter, Susan Bassham, Paul A. Hohenlohe, Eric A. Johnson, William A. Cresko
      Pages 157-178
    3. Martina Bradić, João Costa, Ivo M. Chelo
      Pages 193-210
    4. José A. Andrés, Steven M. Bogdanowicz
      Pages 211-232
  5. Obtaining Candidate Gene Sequences

    1. Front Matter
      Pages 243-243
    2. Patrick T. McGrath
      Pages 257-265

About this book

Introduction

We are entering a particularly fruitful period in evolutionary genetics, as rapid technological progress transforms the investigation of genetic variation within and between species. Molecular Methods for Evolutionary Genetics is a collection of advanced molecular biology protocols and general overviews intended to represent the essential methods currently bringing evolutionary genetics to fruition. Divided into six thematic sections, this volume covers methods for characterizing genomes, diverse approaches to enrich DNA for subsets of the genome prior to sequencing, and state-of-the-art protocols for sampling genetic variation for genetic mapping studies and population genetic studies (RAD sequencing, Sequenom, microarrays, etc.). The volume concludes by focusing on methods to study candidate genes, from obtaining their sequences and analyzing their transcripts to experimentally manipulating their activities in vivo. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Molecular Methods for Evolutionary Genetics serves as a rich resource to biologists interested in evolution, whether they be specialists or beginners in molecular biology.

Keywords

PCR fosmid genomic libraries mRNA microarray phenotypic evolution transcriptome

Editors and affiliations

  • Virginie Orgogozo
    • 1
  • Matthew V. Rockman
    • 2
  1. 1.CNRS UMR7592Université Paris VIIParisFrance
  2. 2.Dept. BiologyNew York UniversityNew YorkUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-228-1
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-227-4
  • Online ISBN 978-1-61779-228-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book