Human Pluripotent Stem Cells

Methods and Protocols

  • Philip H. Schwartz
  • Robin L. Wesselschmidt

Part of the Methods in Molecular Biology book series (MIMB, volume 767)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Laboratory Essentials

    1. Front Matter
      Pages 1-1
    2. Robin L. Wesselschmidt, Philip H. Schwartz
      Pages 3-13
  3. Derivation

    1. Front Matter
      Pages 29-29
    2. Nikolay Turovets, Andrey Semechkin, Leonid Kuzmichev, Jeffrey Janus, Larissa Agapova, Elena Revazova
      Pages 37-54
    3. Hubert E. Nethercott, David J. Brick, Philip H. Schwartz
      Pages 67-85
    4. Knut Woltjen, Riikka Hämäläinen, Mark Kibschull, Maria Mileikovsky, Andras Nagy
      Pages 87-103
  4. Growth, Maintenance, and Expansion

    1. Front Matter
      Pages 105-105
    2. Philip H. Schwartz, David J. Brick, Hubert E. Nethercott, Alexander E. Stover
      Pages 107-123
    3. Rosita Bergström, Susanne Ström, Frida Holm, Anis Feki, Outi Hovatta
      Pages 125-136
    4. Lara J. Ausubel, Patricia M. Lopez, Larry A. Couture
      Pages 147-159
  5. Characterization

    1. Front Matter
      Pages 175-175
    2. Steven E. Bates
      Pages 177-190
    3. Suzanne E. Peterson, Jerold Chun, Jeanne Loring
      Pages 191-200
    4. Hubert E. Nethercott, David J. Brick, Philip H. Schwartz
      Pages 201-220

About this book

Introduction

Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments.  Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better in vitro human disease models; 2) the discovery that a Rho-associated protein Kinase (ROCK) inhibitor allows for efficient single cell passaging and cryopreservation, increasing the efficiency and reliability of hPSC culture; and 3) defined, animal-component-free media, which lay the groundwork for simplified scale-up for therapeutic applications, differentiation protocols, and toxicology screens. The aforementioned technologies can be found in Human Pluripotent Stem Cells: Methods and Protocols, a compilation of 33 detailed protocols in six categories of PSC research that cover laboratory essentials and the derivation of new PSC lines, including induced PSC lines, as well as their growth, maintenance, characterization, genetic manipulation, and differentiation. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

            Authoritative and accessible, Human Pluripotent Stem Cells: Methods and Protocols serves as an ideal guide to scientists conducting their own pluripotent cell research programs and makes great strides towards furthering human knowledge and, ultimately, improving the human condition.

Keywords

cardiomyocytes dopamine neurons episomal transgene expression gene expression analysis human fibroblasts induced pluripotent stem cells nucleofection oligodendrocyte progenitor cells

Editors and affiliations

  • Philip H. Schwartz
    • 1
  • Robin L. Wesselschmidt
    • 2
  1. 1.Research Institute, CHOC Human Neural Stem Cell ResourceChildren's Hospital of Orange CountyOrangeUSA
  2. 2.Beckman Research Institute, Center for Applied Technology DevelopmenCity of HopeDuarteUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-201-4
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-200-7
  • Online ISBN 978-1-61779-201-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book