Nitrogen Fixation

Methods and Protocols

  • Markus W. Ribbe

Part of the Methods in Molecular Biology book series (MIMB, volume 766)

Table of contents

  1. Front Matter
    Pages i-x
  2. Nitrogen Fixation

    1. Front Matter
      Pages 1-1
    2. Yilin Hu, Markus W. Ribbe
      Pages 3-7
    3. Zhi-Yong Yang, Karamatullah Danyal, Lance C. Seefeldt
      Pages 9-29
    4. Chi-Chung Lee, Aaron W. Fay, Jared A. Wiig, Markus W. Ribbe, Yilin Hu
      Pages 31-47
    5. Ina P. O’Carroll, Patricia C. Dos Santos
      Pages 49-65
    6. Jürgen Moser, Markus J. Bröcker
      Pages 67-77
  3. Genetic and Biochemical Methods

    1. Front Matter
      Pages 79-79
    2. Jared A. Wiig, Chi-Chung Lee, Aaron W. Fay, Yilin Hu, Markus W. Ribbe
      Pages 93-103
    3. William E. Newton, Michael J. Dilworth
      Pages 105-127
  4. Spectroscopic Methods

    1. Front Matter
      Pages 145-145
    2. Lauren E. Roth, F. Akif Tezcan
      Pages 147-164
    3. Serena DeBeer
      Pages 165-176
    4. David W. Mulder, John W. Peters
      Pages 177-189
    5. Karamatullah Danyal, Zhi-Yong Yang, Lance C. Seefeldt
      Pages 191-205
    6. Brian J. Hales
      Pages 207-219
    7. Boi Hanh Huynh
      Pages 221-235
  5. Chemical Methods

    1. Front Matter
      Pages 237-237
    2. Aaron W. Fay, Chi-Chung Lee, Jared A. Wiig, Yilin Hu, Markus W. Ribbe
      Pages 239-248
  6. Theoretical Calculations

    1. Front Matter
      Pages 265-265
    2. Travis V. Harris, Robert K. Szilagyi
      Pages 267-291
  7. Back Matter
    Pages 313-326

About this book


Few problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent reduction of atmospheric dinitrogen to bioavailable ammonia.  In Nitrogen Fixation: Methods and Protocols, recognized experts in the field provide an up-to-date, in-depth overview of the methods that have been applied to studying the nitrogenase at a molecular level, ranging from genetic, biochemical, spectroscopic, and chemical methods to theoretical calculations. In addition, techniques used to study an enzyme system that is homologous to nitrogenase are described in this book.  Written in the highly successful Methods in Molecular Biology™ series format, methods chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and cutting-edge, Nitrogen Fixation: Methods and Protocols will be useful for anyone interested in nitrogenase research and willing to venture further toward addressing the remaining mechanistic and biosynthetic questions of this fascinating enzyme system.


Biogenesis Biosynthetic mechanisms Enzymatic mechanisms Genetics Metalloenzyme Nitrogenase Protein biochemistry Spectroscopic techniques Theoretical calculations

Editors and affiliations

  • Markus W. Ribbe
    • 1
  1. 1., Dept Molecular Biology & BiochemistryUniversity of California, IrvineIrvineUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-193-2
  • Online ISBN 978-1-61779-194-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site