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Therapeutic Oligonucleotides

Methods and Protocols

  • John Goodchild

Part of the Methods in Molecular Biology book series (MIMB, volume 764)

Table of contents

  1. Front Matter
    Pages i-xi
  2. John Goodchild
    Pages 1-15
  3. James W. Gaynor, Richard Cosstick
    Pages 17-30
  4. Yoshiyuki Hari, Tetsuya Kodama, Takeshi Imanishi, Satoshi Obika
    Pages 31-57
  5. Laurence Crombez, May C. Morris, Frederic Heitz, Gilles Divita
    Pages 59-73
  6. Samir El Andaloussi, Fatouma Said Hassane, Prisca Boisguerin, Rannar Sillard, Ülo Langel, Bernard Lebleu
    Pages 75-89
  7. Xinrong Liu, Yi Wang, Donald J. Hnatowich
    Pages 91-105
  8. Sigurd Bøe, Lina Prasmickaite, Birgit Engesæter, Eivind Hovig
    Pages 107-121
  9. Masoud M. Toloue, Lance P. Ford
    Pages 123-139
  10. Michael J. Donovan, Ling Meng, Tao Chen, Yunfei Zhang, Kwame Sefah, Weihong Tan
    Pages 141-152
  11. Nikki B. Marshall, Laura L. Hauck, Dan V. Mourich
    Pages 153-168
  12. Angie Cheng, Alexander V. Vlassov, Susan Magdaleno
    Pages 183-197
  13. Angie Cheng, Susan Magdaleno, Alexander V. Vlassov
    Pages 199-213
  14. Emily Dennstedt, Brad Bryan
    Pages 215-222
  15. Rita Bhardwaj, Greice Krautz-Peterson, Patrick J. Skelly
    Pages 223-239
  16. Carmelo Di Primo, Eric Dausse, Jean-Jacques Toulmé
    Pages 279-300
  17. María Luisa Benítez-Hess, Pablo Reyes-Gutiérrez, Luis Marat Alvarez-Salas
    Pages 317-335
  18. Back Matter
    Pages 337-340

About this book

Introduction

The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and nanoparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this exciting and pioneering field.

Keywords

antisense oligonucleotides argonaute protein dinucleotides endothelial cells miRNAs nucleic acid oligodeoxynucleotides oligonucleotide delivery oligoribonucleotides siRNAs tumor cell therapy

Editors and affiliations

  • John Goodchild
    • 1
  1. 1.Dept. ChemistryWorcester State CollegeWorcesterUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-188-8
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-187-1
  • Online ISBN 978-1-61779-188-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site